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ride and drysdale concocted a clever means of assaying chitin in biological samples. Chitin is de-acylated with conc. KOH at high temperatures, precipitated with diatomaceous earth and 75% EtOH, then depolymerized with nitrous acid. After neutralizing the nitrous acid with ammonium sulfamate, the 2,5- anhydromannose can be assayed with MBTH and Iron ion.

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Q: Procedure for the biochemical assay of chitin?
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