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What is the relationship between the absorbance of tryptophan and its concentration in a given solution?
The relationship between the absorbance of tryptophan and its concentration in a solution is direct and proportional. As the concentration of tryptophan in the solution increases, the absorbance of light by the solution also increases. This relationship is described by the Beer-Lambert Law, which states that absorbance is directly proportional to concentration.
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
Why should an absorbance level not exceed a value of 1?
An absorbance value exceeding 1 can indicate that the sample is too concentrated, leading to inaccuracies in the measurement due to light scattering or saturation of the detector. To ensure accurate readings and avoid errors, it is recommended to dilute the sample to bring the absorbance within the linear range of the detector.
What is the theoretical absorbance at 340 nm of a 0.01 M solution of NADH?
The molar absorptivity of NADH at 340 nm is approximately 6,220 M^{-1} cm^{-1}. To calculate the theoretical absorbance, you can use the formula: Absorbance = molar absorptivity x path length x concentration. Given a concentration of 0.01 M and a typical path length of 1 cm, the theoretical absorbance at 340 nm for a 0.01 M solution of NADH would be 0.01 x 6220 x 1 = 62.2 absorbance units.
What are Frequencies of light emitted by an element called?
"Spectrogram" is a word used to describe the set of specific characteristic frequencies of light which are emitted by a given chemical element when it is sufficiently excited by heat or by some other means.Alternatively, a scientific technique known as "spectroscopy" can be used to identify the elements in some matter of unknown composition and also the emission spectra of molecules can be used in chemical analysis of substances.Because each element's emission spectrum is unique, the "emission spectrum" of a chemical element or chemical compound can be used to help identify what it is. The "emission spectrum" is the name given to the relative intensity of each frequency of electromagnetic radiation http://www.answers.com/topic/emission-spectrum by the element's atoms or a compound's molecules when they are returned to a ground state.
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
In a plot of absorbance vs concentration what does the slope represent?
The slope of a plot of absorbance vs. concentration represents the molar absorptivity (also known as the molar absorptivity coefficient or extinction coefficient) of the compound being measured. It indicates how strongly the compound absorbs light at a specific wavelength, and a higher slope indicates a higher absorbance for a given concentration.
What is the relationship between the absorbance of tryptophan and its concentration in a given solution?
The relationship between the absorbance of tryptophan and its concentration in a solution is direct and proportional. As the concentration of tryptophan in the solution increases, the absorbance of light by the solution also increases. This relationship is described by the Beer-Lambert Law, which states that absorbance is directly proportional to concentration.
What is the relationship between the wavelength of light and absorbance in a given substance?
The relationship between the wavelength of light and absorbance in a substance is that different substances absorb light at specific wavelengths. This absorption is measured as absorbance, which increases as the substance absorbs more light at its specific wavelength.
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
You cant figure out what lambda equals when given nu.. v3.2 1012 s-1?
Lambda is equal to the speed of light (3.00 x 10^8) divided by the velocity of the wave.
Inverse exponential distribution?
Where f(x) = lambda* exp(-lambda*x), Inverse cumulative distribution= -ln(1-p)/lambda. See http://en.wikipedia.org/wiki/Exponential_distribution Note that if used in random number generation, with "x" equal to the random deviate, then given U ~ uniform(0,1), then x = -ln(U)/lambda.
What is the common error message that occurs when using the lambda function in Python and providing more arguments than expected?
The common error message that occurs when using the lambda function in Python and providing more arguments than expected is "TypeError: lambda() takes X positional arguments but Y were given."
Why should an absorbance level not exceed a value of 1?
An absorbance value exceeding 1 can indicate that the sample is too concentrated, leading to inaccuracies in the measurement due to light scattering or saturation of the detector. To ensure accurate readings and avoid errors, it is recommended to dilute the sample to bring the absorbance within the linear range of the detector.
Is it possible to calculate the molar extinction coefficient from a single absorbance measurement on one solution of known concentration?
Yes, it is possible to calculate the molar extinction coefficient (ε) from a single absorbance measurement if you have a solution of known concentration. According to Beer-Lambert Law, the relationship is given by A = εcl, where A is the absorbance, c is the concentration, and l is the path length of the cuvette. Rearranging this equation, you can derive ε by using the formula ε = A / (cl), provided you know the absorbance, concentration, and path length.
What is the difference between spectrophotometer and fluorimeter?
A spectrometer shoots light through a sample and detects absorbance while a fluorimeter detects the intensity of fluorescence of a given sample.
What is the theoretical absorbance at 340 nm of a 0.01 M solution of NADH?
The molar absorptivity of NADH at 340 nm is approximately 6,220 M^{-1} cm^{-1}. To calculate the theoretical absorbance, you can use the formula: Absorbance = molar absorptivity x path length x concentration. Given a concentration of 0.01 M and a typical path length of 1 cm, the theoretical absorbance at 340 nm for a 0.01 M solution of NADH would be 0.01 x 6220 x 1 = 62.2 absorbance units.
