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The fidelity of replication is backed by corrective mechanisms to ensure that said replication is a perfect as possible and the same protein structure is being coded for in the daughter cells. Naturally, no process is perfect and mutations will happen, but they are, generally, neutral.

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Marques Hyatt

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2y ago
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14y ago

Mitosis (cell division) is the process that ensures that every new cell in an organism has a full copy of that organism's genetic code. Every cell nucleus has a full copy of the organism's genome and mitosis ensures that all of that genome exists in BOTH daughter cells,.

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12y ago

An intact and error free set of Genetic Instructions that sons and daughters pass along to their offspring.

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14y ago

DNA is replicated during the S phase of mitosis

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11y ago

There are proof-reading and error checking mechanisms in the cells.

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11y ago

The process of DNA replication allows the production of new things (ex: reproduction, the regrown tail of a reptile).

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Q: What does the process of DNA replication ensure?
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What major enzyme involved on replication?

DNA Helicase is the major enzyme involved in the replication of DNA. The reason why it is so important is that it unwinds the DNA which creates two separate strands.


The first step of DNA replication is to copy part of the DNA sequence into?

mRNA


Y shaped regions where the 2 strands of DNA separate?

It is called a replication fork.


What are the steps of DNA replication?

1)The first major step for the DNA Replication to take place is the breaking of hydrogen bonds between bases of the two antiparallel strands. The unwounding of the two strands is the starting point. The splitting happens in places of the chains which are rich in A-T. That is because there are only two bonds between Adenine and Thymine (there are three hydrogen bonds between Cytosine and Guanine). Helicase is the enzyme that splits the two strands. The initiation point where the splitting starts is called "origin of replication".The structure that is created is known as "Replication Fork".2) One of the most important steps of DNA Replication is the binding of RNA Primase in the the initiation point of the 3'-5' parent chain. RNA Primase can attract RNA nucleotides which bind to the DNA nucleotides of the 3'-5' strand due to the hydrogen bonds between the bases. RNA nucleotides are the primers (starters) for the binding of DNA nucleotides.3) The elongation process is different for the 5'-3' and 3'-5' template.a)5'-3' Template: The 3'-5' proceeding daughter strand -that uses a 5'-3' template- is called leading strandbecause DNA Polymerase ä can "read" the template and continuously adds nucleotides (complementary to the nucleotides of the template, for example Adenine opposite to Thymine etc).b)3'-5'Template: The 3'-5' template cannot be "read" by DNA Polymerase ä. The replication of this template is complicated and the new strand is called lagging strand. In the lagging strand the RNA Primase adds more RNA Primers. DNA polymerase å reads the template and lengthens the bursts. The gap between two RNA primers is called "Okazaki Fragments".The RNA Primers are necessary for DNA Polymerase å to bind Nucleotides to the 3' end of them. The daughter strand is elongated with the binding of more DNA nucleotides.4) In the lagging strand the DNA Pol I-exonuclease- reads the fragments and removes the RNA Primers. The gaps are closed with the action of DNA Polymerase (adds complementary nucleotides to the gaps) and DNA Ligase (adds phosphate in the remaining gaps of the phosphate - sugar backbone).Each new double helix is consisted of one old and one new chain. This is what we call semiconservative replication.5) The last step of DNA Replication is the Termination. This process happens when the DNA Polymerase reaches to an end of the strands. We can easily understand that in the last section of the lagging strand, when the RNA primer is removed, it is not possible for the DNA Polymerase to seal the gap (because there is no primer). So, the end of the parental strand where the last primer binds isn't replicated. These ends of linear (chromosomal) DNA consists of noncoding DNA that contains repeat sequences and are called telomeres. As a result, a part of the telomere is removed in every cycle of DNA Replication.6) The DNA Replication is not completed before a mechanism of repair fixes possible errors caused during the replication. Enzymes like nucleases remove the wrong nucleotides and the DNA Polymerase fills the gaps.Similar processes also happen during the steps of DNA Replication of prokaryotes though there are some differences.


What is the first step of DNA replication?

1. Interphase: replication and growth of the chromosomes occur. 2. Prophase: the chromatin condenses to see the individual chromosomes and centrioles move toward the opposite poles of the cell and spindle fibers grow. 3. Metaphase: the chromosomes align in the center of the cell. 4. Anaphase: spindles attach to the chromosomes and split them in part towards the opposite poles. 5. Telophase: the cell begins to separate into to new daughter cells. 6. Cytokinesis: the nucleus begins to form and the cells are completely cut off from each other.