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What enzyme forms covalent bonds between restriction fragments?
DNA ligase forms covalent bonds between restriction fragments by catalyzing the formation of phosphodiester bonds between the sugar-phosphate backbones of adjacent DNA fragments.
How does inorganic phosphate inhibit enzyme activity?
Inorganic phosphate can inhibit enzyme activity by competing with the substrate for the enzyme's active site. This can prevent the substrate from binding to the enzyme and undergoing the catalytic reaction. Additionally, inorganic phosphate may alter the enzyme's conformation, affecting its ability to catalyze the reaction.
What molecule catalyzes covalent bond formation between fragments of DNA?
DNA ligase catalyzes the formation of covalent bonds between fragments of DNA by joining together the sugar-phosphate backbones of adjacent DNA strands. This enzyme plays a critical role in DNA replication, repair, and recombination processes.
How does adding the sodium phosphate solution cause inhibition?
Adding sodium phosphate solution can inhibit enzyme activity by changing the pH of the environment, interfering with the enzyme's structure or binding site, or altering the concentration of ions needed for enzyme function. These changes can disrupt the enzyme-substrate interaction, ultimately decreasing enzyme activity.
What is an enzyme that attaches a phosphate group to another molecule is called?
A kinase is an enzyme that attaches a phosphate group to another molecule through a process known as phosphorylation.
Which enzyme is responsible for creating the covalent bonds that connect the sugar-phosphate backbone of the new DNA molecule?
DNA ligase is the enzyme responsible for creating the covalent bonds that connect the sugar-phosphate backbone of the new DNA molecule during DNA replication and repair processes. It seals the nicks between adjacent nucleotides to form a continuous DNA strand.
Enzyme that analyzes the formation of the sugar to phosphate bonds in DNA?
The enzyme that analyzes the formation of the sugar to phosphate bonds in DNA is DNA polymerase. DNA polymerase is responsible for catalyzing the formation of the phosphodiester bonds between deoxyribose sugars and phosphate groups in the backbone of the DNA molecule during DNA replication.
What enzyme forms covalent bonds between restriction fragments?
DNA ligase forms covalent bonds between restriction fragments by catalyzing the formation of phosphodiester bonds between the sugar-phosphate backbones of adjacent DNA fragments.
How does inorganic phosphate inhibit enzyme activity?
Inorganic phosphate can inhibit enzyme activity by competing with the substrate for the enzyme's active site. This can prevent the substrate from binding to the enzyme and undergoing the catalytic reaction. Additionally, inorganic phosphate may alter the enzyme's conformation, affecting its ability to catalyze the reaction.
How the hydrogen bonds break when the restriction endonuclease enzyme acts on the sugar - phosphate backbone of a DNA?
Restriction endonucleases break hydrogen bonds between complementary base pairs in DNA, not the hydrogen bonds in the sugar-phosphate backbone. These enzymes recognize and bind to specific DNA sequences, then cleave the phosphodiester bonds in the backbone at specific locations, resulting in DNA fragmentation.
Which enzyme is responsible for creating the covalent bonds that connect the sugar phosphate back bone of the new DNA molecules?
An enzyme called HELICASE breaks the sugar to phosphate bonds in DNA strands to initiate DNA replication and DNA transcription.
What molecule catalyzes covalent bond formation between fragments of DNA?
DNA ligase catalyzes the formation of covalent bonds between fragments of DNA by joining together the sugar-phosphate backbones of adjacent DNA strands. This enzyme plays a critical role in DNA replication, repair, and recombination processes.
What enzyme is added to attach the DNA backbone between vector and foreign DNA?
DNA ligase
How does ligase work to join DNA fragments together?
Ligase is an enzyme that helps to join DNA fragments together by forming a strong bond between the sugar-phosphate backbone of the DNA strands. This process is essential for DNA replication, repair, and recombination.
What is the enzyme that convert galactose into glucose 1 phosphate?
The enzyme that converts galactose into glucose 1-phosphate is galactokinase. This enzyme phosphorylates galactose to form galactose 1-phosphate, which can then be converted into glucose 1-phosphate through further metabolic pathways.
What enzyme called is added to attach the DNA backbone between vector and foreign DNA?
DNA ligase
What covalent bonds between the monomers of enzyme macromolecule are?
The covalent bonds between the monomers of enzyme macromolecules are typically peptide bonds. These bonds form between the amino acids in the protein chain through dehydration synthesis, creating a long linear chain that folds into a specific 3D structure necessary for enzyme function.
