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You put in DNA that you want replicated into a buffered solution containing all four nucleotides (AGCT), add primers and Taq Polymerase. Heat to denature the DNA into seperate strands. Then the polymerase will anneal the primers and extend (duplicate the sequence) as cools. Heat... repeat...heat... repeat...etc etc etc until you have as much DNA as you need. 25-35 cycles is usually enough to produce all the DNA you need for most other applications.

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Arjun Bednar

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3y ago

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What happens during an exothermic reaction and how does it differ from an endothermic reaction?

During an exothermic reaction, heat is released to the surroundings, resulting in an increase in temperature. In contrast, an endothermic reaction absorbs heat from the surroundings, causing a decrease in temperature.


What are the steps to a chain reaction?

A chain reaction typically involves three steps: initiation, propagation, and termination. During initiation, the reaction is triggered by an initial event. In propagation, products from the initial reaction continue to react, creating more products. Termination occurs when the reaction eventually stops due to the depletion of reactants or other factors.


How is the chain reaction in a nuclear reactor different from one in an atomic bomb?

In a nuclear reactor, the chain reaction is controlled to produce a steady flow of energy by regulating the rate of reactions. In an atomic bomb, the chain reaction happens rapidly and uncontrollably, resulting in a massive release of energy in a short period of time, leading to an explosion.


Which pair of functional groups can be part of a condensation reaction form a polymer chain?

The pair of functional groups that can be part of a condensation reaction to form a polymer chain is hydroxyl (-OH) and carboxyl (-COOH) groups. The hydroxyl group provides the -OH to form water during the condensation reaction, linking monomers together to form the polymer chain.


What role do nucleotides play in the polymerase chain reaction (PCR)?

Nucleotides serve as the building blocks for creating new DNA strands during the polymerase chain reaction (PCR). They are incorporated by the DNA polymerase enzyme to extend the DNA strands, allowing for the amplification of specific DNA sequences.

Related Questions

Which describes what happens during a chain reaction?

Neutrons released during a fission reaction trigger other fission reactions.


What happens during a chain reaction and how does it impact the surrounding environment?

During a chain reaction, a series of reactions occur in which the products of one reaction serve as the reactants for the next reaction. This can lead to a rapid and uncontrollable release of energy, such as in a nuclear chain reaction. The impact on the surrounding environment can be significant, as it can result in the release of harmful radiation, heat, and potentially radioactive materials, causing damage to living organisms and the ecosystem.


What happens in a chain reaction automobile accident?

In a chain reaction automobile accident one crash causes multiple one. For example, when car a hits car b, car b moves forward and hits car c. This reaction happens with multiple cars and can be dangerous.


The subatonic particles released during a nuclear chain reaction are what?

subatonic particles


What happens to the potential of the molecules in a reaction?

the potential energy of the molecules changes during a reaction.


What happens during an irreversible chemical reaction?

~*cock block*~


What happens to the potential energy of the molecule in the reaction?

The potential energy of the molecules change during a reaction.


What is the duration of The Chain Reaction?

The duration of The Chain Reaction is 1.53 hours.


What are used to show what happens during a chemical reaction?

Chemical equations are used to show what happens during a chemical reaction. They demonstrate the reactants that are used, the products that are formed, and the balanced relationship between them. Symbols and formulas represent the different elements and compounds involved in the reaction.


What happens to the original substance during a chemical reaction?

The original substances are transformed during a chemical reaction a new substance (compound) is obtained.


What happens during the light reaction?

glucose is formed in the form of ATP


What are used to show happens during a chemical reaction?

CHEMiCAL SyMBOlS