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What happens when you prepare a smear from a agar plate or slant without first placing water on slide?

If you prepare a smear from an agar plate or slant without first placing water on the slide, the cells may not adhere well to the slide, leading to uneven distribution and difficulty in visualization. Adding a drop of water before preparing the smear helps the cells adhere to the slide and spread evenly for better microscopic examination.


Why tap water is used for smear washing in staining?

Tap water is used to wash the excess stain from a slide prepared from a smear. You can use tap water instead of distilled water because you aren't worried about a precipitate forming and tap water is much cheaper than distilled.


Is presence of grease or dirt on a glass slide going to result in poor smear preparation?

The smear will not adhere well if there is grease on the slide.


Why is there need to fix the bacteria to the slide?

To heat fix a bacterial smear you would put the specimen on the slide and either put slide on a slide warmer or over a Bunsen burner for a few seconds. Heat fixing a bacterial smear does kill the specimen but it makes the bacteria stick the slide to withstand the rinsing process.


What precautions are important when a prepared slide is observed under the microscope?

Microscopes work on the principle of magnifying light rays passing through a tiny object. The object should be transparent or translucent and colored to be properly viewed under a compound microscope. Some of the mandatory requirements to prepare a good slide for viewing are as follows: Before creating a smear, always check that the slide is clean and perfectly transparent. It should also be microbe free. So, take a slide and wash it first with soap-water, and then wipe it with ethanol (ethyl alcohol). This makes the slide clean and sterilized. With regard to preparing a slide of bacterial specimen, when you create a smear, do not make a thick layer of smear. Take very little quantity of the inoculum. If by chance you take too much of the inoculum, spread it over the slide to a larger area, such as to avoid a thick smear. Air drying is necessary, as it lets the bacteria congregate at their places. Heat fix the slide with precision. Too much heat fix can kill the organism, and too little of it will make the organisms too loosely bound to the slide surface. If they are loosely bound, they will fall off when you flood the slide with stain. One good way of identifying the extent of heat fix is to feel it after you pass the slide through the flame. It should neither be too hot nor warm. It should give you the sense of heat but tolerable. When you stain the slide, do not stain the whole surface of the slide. This wastes much of the stain and is messy. Cleanliness is very important in all science experiments. Just staining the area containing the smear is enough. Usually, stainings are done for a minute or two, but for certain experiments like endospore staining, the extent of staining time may be as long as 10 mins or even more. During such cases, ensure that the stain does not dry over the smear. Maintain liquidity of the stain, as it is to be washed after some time. While washing the slide after staining, do not let the water stream fall directly on the smear. This may disrupt the smear. Let the stream of water flow slowly along the surface, such that only the stain is flooded and the smear is intact. While preparing fungal slides, take the stain first, and then the hyphal fragments. Crush the hyphal fragments properly by placing a coverslip over the fragments (avoiding air bubbles) and the slowly tapping it with the butt of a pencil. Always observe under 10X first. This will give you an idea of the location of a good area for observation. After this, you may prefer to switch over to 45X. 100X objective in compound microscopes is always used as an oil-immersion objective, so do not ever observe at a specimen at 100X without oil.


What happens when you put to much cat food and you cant slide it on poptropica shrink ray island?

Restart the island boring!


What happens if to much water is in a cell?

Too much water inside a cell will cause it to burst.


How do you use trombone slide cream?

Apply a liberal amount of cream to the slide, then spread it around by moving your slide up and down. Apply a thin coating of water to the slide, and you're good to go. Make sure not to use too much slide cream.


How much water does a water slide need to operate?

The amount of water a water slide needs to operate can vary widely depending on the slide's design, length, and size. Generally, a typical water slide may require anywhere from 50 to 200 gallons of water per minute to provide sufficient flow and ensure a smooth ride. Larger, more complex slides may need even more water to maintain proper operation and safety. Additionally, pools or catch basins at the slide's base might help recirculate water, affecting the overall water requirement.


What happens to a baby dolphin when it spends too much time on the surface of the water?

what happens when a baby dolphin spends too much time on the surface of the water


What happens when hot water meets cold water?

cold water is much heavier than hot water . the water becomes warm


Why are thick or dense smears less likely to provide a good smear preparation?

because if too much smear the sample will look to indistinct