The slide can get damaged.
Ballpoint pens smear because the ink takes time to dry on the surface of the paper. When the ink is still wet, it can easily transfer onto hands or smear across the paper when brushed against. Factors like paper quality and writing speed can also affect how much a ballpoint pen smears.
If there is too much calcium in your pool water, it can lead to scaling on pool surfaces and equipment. This can affect water flow in pipes, reduce the effectiveness of sanitizer, and create cloudy water. It is recommended to maintain calcium hardness levels within the recommended range to prevent these issues.
If you evaporate 10 grams of salt in 50 ml of water, all of the salt will remain once the water has completely evaporated. Evaporation only removes the water, leaving behind the salt.
When salt is added to water, the salt particles dissolve and break apart into ions, increasing the density of the water. This makes the water more conductive, changes its freezing and boiling points, and affects the taste.
Diffusion happens at a faster in hotter temperature of water than a colder temperature of water because the particles with have more kinetic energy, allowing them to move much quicker from high concentration to low.
If you prepare a smear from an agar plate or slant without first placing water on the slide, the cells may not adhere well to the slide, leading to uneven distribution and difficulty in visualization. Adding a drop of water before preparing the smear helps the cells adhere to the slide and spread evenly for better microscopic examination.
Tap water is used to wash the excess stain from a slide prepared from a smear. You can use tap water instead of distilled water because you aren't worried about a precipitate forming and tap water is much cheaper than distilled.
The smear will not adhere well if there is grease on the slide.
To heat fix a bacterial smear you would put the specimen on the slide and either put slide on a slide warmer or over a Bunsen burner for a few seconds. Heat fixing a bacterial smear does kill the specimen but it makes the bacteria stick the slide to withstand the rinsing process.
Microscopes work on the principle of magnifying light rays passing through a tiny object. The object should be transparent or translucent and colored to be properly viewed under a compound microscope. Some of the mandatory requirements to prepare a good slide for viewing are as follows: Before creating a smear, always check that the slide is clean and perfectly transparent. It should also be microbe free. So, take a slide and wash it first with soap-water, and then wipe it with ethanol (ethyl alcohol). This makes the slide clean and sterilized. With regard to preparing a slide of bacterial specimen, when you create a smear, do not make a thick layer of smear. Take very little quantity of the inoculum. If by chance you take too much of the inoculum, spread it over the slide to a larger area, such as to avoid a thick smear. Air drying is necessary, as it lets the bacteria congregate at their places. Heat fix the slide with precision. Too much heat fix can kill the organism, and too little of it will make the organisms too loosely bound to the slide surface. If they are loosely bound, they will fall off when you flood the slide with stain. One good way of identifying the extent of heat fix is to feel it after you pass the slide through the flame. It should neither be too hot nor warm. It should give you the sense of heat but tolerable. When you stain the slide, do not stain the whole surface of the slide. This wastes much of the stain and is messy. Cleanliness is very important in all science experiments. Just staining the area containing the smear is enough. Usually, stainings are done for a minute or two, but for certain experiments like endospore staining, the extent of staining time may be as long as 10 mins or even more. During such cases, ensure that the stain does not dry over the smear. Maintain liquidity of the stain, as it is to be washed after some time. While washing the slide after staining, do not let the water stream fall directly on the smear. This may disrupt the smear. Let the stream of water flow slowly along the surface, such that only the stain is flooded and the smear is intact. While preparing fungal slides, take the stain first, and then the hyphal fragments. Crush the hyphal fragments properly by placing a coverslip over the fragments (avoiding air bubbles) and the slowly tapping it with the butt of a pencil. Always observe under 10X first. This will give you an idea of the location of a good area for observation. After this, you may prefer to switch over to 45X. 100X objective in compound microscopes is always used as an oil-immersion objective, so do not ever observe at a specimen at 100X without oil.
Restart the island boring!
Too much water inside a cell will cause it to burst.
Apply a liberal amount of cream to the slide, then spread it around by moving your slide up and down. Apply a thin coating of water to the slide, and you're good to go. Make sure not to use too much slide cream.
The amount of water a water slide needs to operate can vary widely depending on the slide's design, length, and size. Generally, a typical water slide may require anywhere from 50 to 200 gallons of water per minute to provide sufficient flow and ensure a smooth ride. Larger, more complex slides may need even more water to maintain proper operation and safety. Additionally, pools or catch basins at the slide's base might help recirculate water, affecting the overall water requirement.
what happens when a baby dolphin spends too much time on the surface of the water
cold water is much heavier than hot water . the water becomes warm
because if too much smear the sample will look to indistinct