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What precautions are important when a prepared slide is observed under the microscope?
Microscopes work on the principle of magnifying light rays passing through a tiny object. The object should be transparent or translucent and colored to be properly viewed under a compound microscope. Some of the mandatory requirements to prepare a good slide for viewing are as follows:
Before creating a smear, always check that the slide is clean and perfectly transparent. It should also be microbe free. So, take a slide and wash it first with soap-water, and then wipe it with ethanol (ethyl alcohol). This makes the slide clean and sterilized.
With regard to preparing a slide of bacterial specimen, when you create a smear, do not make a thick layer of smear. Take very little quantity of the inoculum. If by chance you take too much of the inoculum, spread it over the slide to a larger area, such as to avoid a thick smear.
Air drying is necessary, as it lets the bacteria congregate at their places. Heat fix the slide with precision. Too much heat fix can kill the organism, and too little of it will make the organisms too loosely bound to the slide surface. If they are loosely bound, they will fall off when you flood the slide with stain. One good way of identifying the extent of heat fix is to feel it after you pass the slide through the flame. It should neither be too hot nor warm. It should give you the sense of heat but tolerable.
When you stain the slide, do not stain the whole surface of the slide. This wastes much of the stain and is messy. Cleanliness is very important in all science experiments. Just staining the area containing the smear is enough. Usually, stainings are done for a minute or two, but for certain experiments like endospore staining, the extent of staining time may be as long as 10 mins or even more. During such cases, ensure that the stain does not dry over the smear. Maintain liquidity of the stain, as it is to be washed after some time.
While washing the slide after staining, do not let the water stream fall directly on the smear. This may disrupt the smear. Let the stream of water flow slowly along the surface, such that only the stain is flooded and the smear is intact.
While preparing fungal slides, take the stain first, and then the hyphal fragments. Crush the hyphal fragments properly by placing a coverslip over the fragments (avoiding air bubbles) and the slowly tapping it with the butt of a pencil.
Always observe under 10X first. This will give you an idea of the location of a good area for observation. After this, you may prefer to switch over to 45X.
100X objective in compound microscopes is always used as an oil-immersion objective, so do not ever observe at a specimen at 100X without oil.
What else can I help you with?
What is an Henrici slide?
A Henrici slide is a specialized microscope slide used in microbiology to examine and identify fungi. It consists of a thin layer of agar on a glass slide, which allows fungi to be cultured and observed under the microscope. It is named after the Swiss mycologist, Hanns Hofmann Henrici.
Are samples for compound light microscope prepared on glass slides?
Yes
what microscope shows 3 dimensional images?
A scanning tunneling microscope can show detailed surface structures but the organism must be prepared by being plated with a very thin layer of metal. Most organisms can't survive this process. To see a three dimensional image of a living organism you should select a stereo microscope.
Michael took a swab of bacteria from a Petri dish and placed it on a glass slide. Then he treated the sample with a series of dyes. For which type of microscope is the sample prepared?
The sample is prepared for a light microscope. The application of dyes enhances the contrast of the bacteria, making them more visible when viewed under light microscopy. This technique is commonly used in microbiology to differentiate and identify bacterial species based on their staining characteristics.
Can you see protozoan in wet mount stool sample?
Yes, protozoa can be observed in a wet mount stool sample, particularly if the sample is fresh and properly prepared. Wet mounts allow for the visualization of microorganisms, including protozoan cysts or trophozoites, under a microscope. However, the ability to see them depends on the concentration and viability of the organisms in the sample. Staining techniques may enhance visibility for more accurate identification.
Why is it important for the slide to be prepared so that light passes through it?
If your microscope is a Transmission Microscope then light has to pass through the specimen (that is how the microscope works).Other kinds of microscopes may not require this.
What is an Henrici slide?
A Henrici slide is a specialized microscope slide used in microbiology to examine and identify fungi. It consists of a thin layer of agar on a glass slide, which allows fungi to be cultured and observed under the microscope. It is named after the Swiss mycologist, Hanns Hofmann Henrici.
Is sleeping outside dangerous?
Sleeping outside can be dangerous due to exposure to the elements, wildlife, and potential safety risks. It is important to take precautions and be prepared when sleeping outdoors.
Are samples for compound light microscope prepared on glass slides?
Yes
Why is it important to be prepared for an experiment by taking the appropriate safety precautions?
Being prepared for an experiment by taking the appropriate safety precautions is crucial to prevent accidents and ensure the safety of all participants. Proper safety measures help minimize risks associated with hazardous materials and equipment, protecting both individuals and the environment. Additionally, being prepared fosters a more focused and effective research environment, allowing for reliable results without the distraction of managing unexpected incidents. Ultimately, safety precautions are essential for ethical and responsible scientific practice.
Why is it important that a specimen for an specimen microscope be placed in a vacuum Why is this step unnecessary for a light microscope?
Placing a specimen in a vacuum is essential for an electron microscope because it prevents air molecules from scattering electrons, which would otherwise degrade the image quality and resolution. In contrast, light microscopes use visible light, which can travel through air without interference, making a vacuum unnecessary. Moreover, biological specimens are often observed in their natural state with light microscopy, while electron microscopy requires samples to be prepared and often coated to withstand the vacuum environment.
How must samples be prepared for observation by an electron microscope?
Samples for observation by an electron microscope are typically prepared by fixing the specimen in a chemical solution, dehydrating it, and then embedding it in a resin for stability. The sample is then sliced into ultrathin sections using a microtome before being stained to enhance contrast. Finally, the prepared sample is placed inside the electron microscope for imaging.
What kind of microscope uses two or more glass lenses to magnify either living cells or prepared slides?
A compound microscope uses two or more glass lenses to magnify either living cells or prepared slides. This type of microscope is commonly used in laboratories and educational settings to study cells and microorganisms at a cellular level.
How is extracted breast milk prepared for laboratory testing?
The milk is cultured, allowing colonies of bacteria to grow. The causative bacteria then can be specially prepared for identification under a microscope.
How must samples be prepared by observation by an electron microscope?
They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.
XeOF4 have been prepared from Xenon but not the XeO2F4 Why?
XeOF4 is prepared by reacting xenon hexafluoride with oxygen. On the other hand, XeO2F4 is not directly prepared from xenon as it is a hypothetical compound that has not been experimentally observed or synthesized.
What is a prepared slide on a micro scope?
A prepared slide for a compound light microscope is composed of a viewing sample suspended in a drop of distilled water, which has been placed on one glass slide. A second glass slide will be placed on the first slide and any residual water will be gently soaked up by a paper towel.
