0
Absorbance refers to a measure of the capacity associated with a substance as regards absorption of light of a specified wavelength. Whenever you plot a graph of absorbance vs. concentration a direct relationship should be produced
Wiki User
∙ 9y ago
Wiki User
∙ 11y agoIf I have a purified protein with a extinsion coeficiente of 1.3450 and absorbance of 2.1491 AU, what is the correct formula to determine the protein concentation (mg/mL)?
Add your answer:
Earn +20 pts
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
Why should the y intersect of any Beer's law plot equal zero?
Beer's law says that absorbance is directly proportional to concentration. So if there is zero concentration there should be zero absorbance (0,0).
What is the relation of Beers Lambert's law in UV Visible spectrometer?
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
Does absorbance increase or decrease as the solution concentration of the substance increase?
The light is absorbed at a specific wavelength and this is because the electrons in the chemical bonds only absorb certain wavelengths of light. So, more concentration, more bonds and more electrons to absorb the light.
Does Amax of bromophenol blue vary with concentration?
Bromophenol Amax or maximum absorbance in spectrophotometer is 590nm I guess so hehe^^ based on my experimental result though!
In a plot of absorbance vs concentration what does the slope represent?
The slope of absorbance vs concentration reptresents the value of εb, where ε is the absorbtivity with units of (L/mol cm) and b is path length measured in cm.
Is there a simple equation to calculate the concentration given the absorbance and a time?
You need a graphic concentration versus absorbance.
How to calculate lambda max?
using uv-visible spectrophotometer concentration vs absorbance is plotted and the maximum absorbance of the drug is lambda max of the drug. then after it will decrease. still if needed clarification, refer beer lambert"s law
The absorbance of a solution of NiCl2 of unknown concentration is found to be 0.388 what is the concentration of this soultion?
684
What is the formula that use to calculate the concentration of glucose in blood by the principle of Beer-Lambert law and application of glucose oxidase?
patients absorbance/absorbance of the standard*concentration of the standard gives you the glucose concentration of the patients sample
What is external calibration and is it the same as the manual calibration of equipment?
Well, external calibration is a method used in analytical chemistry to determine the concentration of an unknown analyte. In essence, you take known concentrations of the analyte and plot it on an absorbance or transmittance graph to get a linear plot. And then you take that linear equation and plug in the absorbance or transmittance value received from the unknown solution and get the concentration. An example of this is if you want to find out the amount of calcium in a vitamin tablet. Dissolve the vitamin tablet and test the solution to get an absorbance value. Then test by the same method various concentrations of a calcium solution, plot this on a graph of absorbance vs. concentration and there yah go.
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
Why do chemists prefer to read the absorbance rather than the percent transmittance of lght when analyzing a sample having a visible color?
Because when you graph a molar concentration vs. absorbance graph, the graph is linear, making the graph easier to read.
Why should the y intersect of any Beer's law plot equal zero?
Beer's law says that absorbance is directly proportional to concentration. So if there is zero concentration there should be zero absorbance (0,0).
Why Beer's law is obeyed at low concentration and not at high concentration?
Because in high concentrations you will have scattering, multi event absorbance, and self-absorbance which are mostly non-linear effects and are not described by the Beer's law.
Is absorbance depends on the temp of the room or solvents in spectrophotometric measurement?
The absorbance depends on the concentration of the chemical species to be analyzed (for identic spectrophotometric cells).
How the absorbance data correlates to the initial protein concentration in ug ml?
The absorbance data correlates to the initial protein concentration in ug ml in one way. It breaks down protein in the stomach by the action of the stomach acid.
