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Its a reagent used to determine the glucose content of a sample. its based on 2 coupled enzyme reactions with a colorimetric end-point:
D-glucose + O2 + H2O ----- glucose oxidase ------> H2O2 + gluconate
aminophenazone + phenol + H2O2 -----peroxidase----> a red dye + H2O
(NOTE: H2O2 product of 1st reaction acts as substrate for 2nd)
this red coloured sample can then be put into a spectrophotometer and an absorbency reading can be taken, this reading can be compared to a calibration curve and the content of glucose can be ascertained.
What else can I help you with?
What does the acronym chod-pap in cholesterol testing stand for?
CHOD-PAP stands for cholesterol oxidase-phenol and aminophenazone. These are reagents used in enzymatic methods for measuring cholesterol levels in blood samples.
What happens to glucose in the phenol sulfuric acid test?
In the phenol sulfuric acid test, glucose reacts with phenol and sulfuric acid to form a colored product. The color change is due to the formation of furfural, which reacts with the sulfuric acid to produce a colored compound. This color change is used to detect the presence of glucose in a sample.
Can you get phenol from carbolic acid?
Phenol and carbolic acid are actually the same compound. "Carbolic acid" is an older, colloquial term for phenol.
Phenol red turns back to red when a plant in light is added to the solution in terms of the reaction why does this occur?
This occurs because plants undergo photosynthesis in the presence of light, utilizing carbon dioxide and water to produce glucose and oxygen. The glucose produced by the plant may lower the pH of the solution, causing the phenol red indicator to shift back to red.
What increase absorbance of phenol in ethanol?
Possible factors that can increase the absorbance of phenol in ethanol are: increasing the concentration of phenol in the solution, using a higher path length cuvette for measurement, and selecting a wavelength for measurement where phenol has a higher molar absorptivity coefficient.
What does the acronym chod-pap in cholesterol testing stand for?
CHOD-PAP stands for cholesterol oxidase-phenol and aminophenazone. These are reagents used in enzymatic methods for measuring cholesterol levels in blood samples.
What enzyme is present in liver and potatoes?
Catalase is the most well known enzyme common to both liver and potato, but there are others enzymes that can be found in potato. Oxidase and peroxidase are the other enzymes that can be found in potato. Oxidases in potato includes tyrosinase, cathecol oxidase and cytochrome oxidase. Peroxidase have the same function as with oxidase but they need a co-factor in order to complete its required action. The co-factor phenol oxidase is found abundant in potatoes. Hence, it will have a positive reaction once a substrate will be added indicating the presence of peroxidase in potatoes.
What are the products and reactants in an browning apple?
Browning of the apples is caused by the oxidation of the enzyme phenol oxidase:phenol oxidase + oxygen---------------melanins
What happens to glucose in the phenol sulfuric acid test?
In the phenol sulfuric acid test, glucose reacts with phenol and sulfuric acid to form a colored product. The color change is due to the formation of furfural, which reacts with the sulfuric acid to produce a colored compound. This color change is used to detect the presence of glucose in a sample.
Poly phenol oxidase enzyme?
Translate text or webpage <span class="cs_misspelled">Polyphenol</span> <span class="cs_misspelled">oxidase</span> <span class="cs_misspelled">(tyrosinase)</span> Translate from: English Type text or a website address or translate a document. Cancel Example usage of "": automatically translated by Google Persian English Spanish Alpha Discovered the enzyme polyphenol oxidase (tyrosinase)
Why should peeled potatoes not to be exposed to air longer then necessary?
peeled potatoes should not be exposed to air longer than necessary to avoid oxidation and changing of color of the surface. it contains the polyphenol oxidase which oxidizes catechol to o-diphenol. the phenol oxidases are copper containing proteins which can oxidize phenol derivatives to quinones.
What is the reaction result for the methy red test for glucose on Staphylococcus epidermidis?
In my microbilogy lab, it was Glucose negative (meaning no gas or acid produced.) We used Phenol Red, however.
What is the function of polyphenol oxidase in plants?
Polyphenol oxidase is an enzyme that catalyzes the oxidation of phenols to quinones. This enzymatic reaction is responsible for browning reactions in plants when tissues are damaged, such as in fruit browning. It also plays a role in plant defense against pathogens and pests by producing toxic compounds.
Structure of phenol?
phenol
What is the chemistry of the CHOD-PAP method of cholesterol measurement?
Chemistry of the CHOD-PAP method of cholesterol measurementThe cholesterol is determined after enzymatic hydrolysis and oxidation.The indicator quinoneimine is formed from hydrogen peroxide and4-aminoantipyrine in the presence of phenol and peroxidase.CholesterolCholesterol ester + H2O ----------------------> Cholesterol + Fatty acidsesteraseCholesterolCholesterol + O2 ----------------------------->Cholestene-3-one + H2O2oxidasePeroxidase2H2O2 + phenol + 4-Aminoantipyrine ---------------------------> quinoneimine + 4H2O
What is the derivative of Phenol?
The derivative of Phenol is Carbolic acid.
Does phenol react with bromine?
Yes, at room temperature bromine reacts with phenol and forms 2,4,6-tribromo phenol.
