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Reverse hybridization assays offer a platform for highly specific probe hybridization.

Specific DNA probes are immobilized on a solid carried, such as nitrocellulose strips or Luminex beads.

The test procedure comprises three parts:

  • Isolation of the DNA from the sample (reagents NOT provided in the kit)
  • Amplification of target DNA using PCR
  • Detection of the biotinylated product in our reverse hybridization assays
DNA or RNA derived amplification products can be denatured and hybridized to the immobilized probes. After stringent washing steps, the specific hybrids can be detected. On reverse hybridization strips, this results in a visible hybridization pattern. In the Luminex assay format, this can be measured by a specific Luminex reader.

Reverse hybridization assays have the following advantages:

  • Hybridization is highly specific, allowing single nucleotide mismatch detection
  • Detection is very sensitive, especially to detect minority species amplimers e.g. in mixed infections
  • The read-out can be performed manually or automated
  • The test is fast (about 8 hours, including amplification)
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What is the hybridization of NCl3?

The hybridization of NCl3 is sp3.


What is hybridization of Be in BeH2?

The hybridization of Be in BeH2 is sp hybridization. Beryllium has 2 valence electrons and forms 2 bonds with the two hydrogen atoms in BeH2, resulting in sp hybridization.


What is the hybridization of Alkyne?

The hybridization of the carbon atoms in an alkyne is sp.


How can one determine the hybridization of an atom from its Lewis structure?

To determine the hybridization of an atom from its Lewis structure, count the number of electron groups around the atom. The hybridization is determined by the number of electron groups, with each group representing a bond or lone pair. The hybridization can be identified using the following guidelines: If there are 2 electron groups, the hybridization is sp. If there are 3 electron groups, the hybridization is sp2. If there are 4 electron groups, the hybridization is sp3. If there are 5 electron groups, the hybridization is sp3d. If there are 6 electron groups, the hybridization is sp3d2.


How can one determine the hybridization of an atom in a molecule based on its Lewis structure?

To determine the hybridization of an atom in a molecule based on its Lewis structure, count the number of electron groups around the atom. The hybridization is determined by the number of electron groups, with each group representing a bond or lone pair. The hybridization can be determined using the following guidelines: 2 electron groups: sp hybridization 3 electron groups: sp2 hybridization 4 electron groups: sp3 hybridization 5 electron groups: sp3d hybridization 6 electron groups: sp3d2 hybridization

Related Questions

What is the hybridization of NCl3?

The hybridization of NCl3 is sp3.


What is hybridization of Be in BeH2?

The hybridization of Be in BeH2 is sp hybridization. Beryllium has 2 valence electrons and forms 2 bonds with the two hydrogen atoms in BeH2, resulting in sp hybridization.


What is the hybridization of Alkyne?

The hybridization of the carbon atoms in an alkyne is sp.


How can one determine the hybridization of an atom from its Lewis structure?

To determine the hybridization of an atom from its Lewis structure, count the number of electron groups around the atom. The hybridization is determined by the number of electron groups, with each group representing a bond or lone pair. The hybridization can be identified using the following guidelines: If there are 2 electron groups, the hybridization is sp. If there are 3 electron groups, the hybridization is sp2. If there are 4 electron groups, the hybridization is sp3. If there are 5 electron groups, the hybridization is sp3d. If there are 6 electron groups, the hybridization is sp3d2.


How can one determine the hybridization of an atom in a molecule based on its Lewis structure?

To determine the hybridization of an atom in a molecule based on its Lewis structure, count the number of electron groups around the atom. The hybridization is determined by the number of electron groups, with each group representing a bond or lone pair. The hybridization can be determined using the following guidelines: 2 electron groups: sp hybridization 3 electron groups: sp2 hybridization 4 electron groups: sp3 hybridization 5 electron groups: sp3d hybridization 6 electron groups: sp3d2 hybridization


How can one determine the orbital hybridization of an atom in a molecule?

To determine the orbital hybridization of an atom in a molecule, you can look at the atom's steric number, which is the sum of the number of bonded atoms and lone pairs around the atom. The hybridization is determined by the steric number according to the following guidelines: Steric number 2: sp hybridization Steric number 3: sp2 hybridization Steric number 4: sp3 hybridization Steric number 5: sp3d hybridization Steric number 6: sp3d2 hybridization By identifying the steric number, you can determine the orbital hybridization of the atom in the molecule.


What is the hybridization of N in N2?

The hybridization of N i n N2 is sp.


Which is the hybridization of a carbon with a linear structure and an angle of 180º?

sp hybridization.


When was Experiments on Plant Hybridization created?

Experiments on Plant Hybridization was created in 1865.


What is the hybridization of the boron atom in BF3?

The boron atom in BF3 has sp2 hybridization.


What is the hybridization of the carbon atom in CF4?

The carbon atom in CF4 has a hybridization of sp3.


What is the hybridization of the central atom in NCl3?

The hybridization of the central atom in NCl3 is sp3.