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Some viva voce questions for electrophoresis?

1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).


Does gel electrophoresis separate molecules based on their charge?

Yes, gel electrophoresis separates molecules based on their size and charge.


What color tube is drawn for a hgb electrophoresis?

A lavender-top (EDTA) tube is typically used for drawing blood samples for hemoglobin electrophoresis testing.


Which pieces of DNA will migrate to the bottom of the DNA gel first during electrophoresis?

During electrophoresis, smaller pieces of DNA will migrate to the bottom of the gel first.


What color test tube for protein electrophoresis?

For protein electrophoresis, a clear or colorless test tube is typically used. This allows for easy visualization of the protein bands after electrophoresis is complete. Any other colored test tube could interfere with accurate observation and analysis of the results.

Related Questions

What are the application of paper electrophoresis?

Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.


What was used before gel electrophoresis?

Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.


Similarities between gel electrophoresis and paper chromatography?

nothing


Some viva voce questions for electrophoresis?

1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).


Example of a definition of terms in a research paper?

example of definition of terms


What is the definition of paper size?

The standardised sizes of commercially used paper.


What has the author A J Houtsmuller written?

A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins


When was Electrophoresis - journal - created?

Electrophoresis - journal - was created in 1980.


What is the definition of 2d drawing?

the definition of 2D is it is flat on the piece of paper -A design


What is a common name for gel electrophoresis?

Agarose gel electrophoresis.


What is the definition of the term quire?

The definition of the term 'quire' is: any collection of paper leaves in a manuscript or book. It can also mean to fold four sheets of parchment paper into eight leaves of paper.


What has the author B J Haywood written?

B. J. Haywood has written: 'Electrophoresis - technical applications' -- subject(s): Abstracts, Bibliography, Electrophoresis 'Electrophoresis-technical application' -- subject(s): Bibliography, Electrophoresis