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The reaction between egg albumin, potassium nitrate, and sodium carbonate would not result in a single equation since egg albumin is a complex mixture of proteins. Each component would likely undergo different reactions when mixed with potassium nitrate and sodium carbonate. It would be necessary to isolate and identify the specific components of the egg albumin that are reacting with the potassium nitrate and sodium carbonate to write a balanced chemical equation for the overall reaction.

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A positive reaction for albumin in the biuret test occurs because albumin contains peptide bonds, which the biuret reagent detects. The reagent contains copper ions that bind to these peptide bonds, resulting in a color change, typically to violet or purple. This reaction indicates the presence of proteins, specifically those containing multiple peptide bonds, like albumin. Therefore, a positive biuret test confirms the presence of protein in the sample.


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Albumin reacts with concentrated nitric acid by undergoing nitration, where nitrogen atoms from the nitrate ions in the acid are added to the protein. This reaction can lead to denaturation of the albumin protein, resulting in loss of its biological activity and potential degradation of its structure.


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