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potassium nitrate

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Protocol for nitrate reductase using sodium nitrite as standard?

To measure nitrate reductase activity using sodium nitrite as the standard, you would need to create a standard curve by plotting the absorbance values of known concentrations of nitrite against their concentrations. Then, you can measure the nitrate reductase activity by monitoring the decrease in nitrite concentration over time in the presence of the enzyme. The activity can be calculated based on the slope of the standard curve and the decrease in nitrite concentration in the enzyme reaction.


What is the chemical reaction catalyzed by nitrate reductase?

Nitrate reductase catalyzes the reduction of nitrate (NO3-) to nitrite (NO2-). This reaction involves the transfer of electrons to convert nitrate into nitrite, an important step in the nitrogen cycle.


Which of the not a prosthetic group of nitrate reductase?

Nitrate reductase does not contain the prosthetic group heme. Instead, it typically contains molybdenum cofactor (Moco) and heme iron-sulfur center as prosthetic groups.


What has the author Terence A Walsh written?

Terence A. Walsh has written: 'Studies on the nitrate reductase from Pseudomonas aeruginosa'


What has the author Cynthia May Lewis written?

Cynthia May Lewis has written: 'Genetic and biomedical studies of regulation of nitrate reductase in Ustilago maydis'


What happens to nitrate when you electroplate sliver nitrate?

When silver nitrate is electroplated, the silver ions in the silver nitrate solution are reduced at the cathode, forming a silver metal coating on the substrate. The nitrate ions from the silver nitrate solution are left in the solution without participating directly in the electroplating process.


What has the author Claudio Lovato written?

Claudio Lovato has written: 'Nitrate reductase activity in leaves and its relationship to nitrogen yield in four spring barley (Hordeum vulgare L.) cultivars' -- subject(s): Barley


Which protein blocks the active site?

In enzymatic reactions, competitive inhibitors are proteins or molecules that block the active site of an enzyme, preventing substrate binding. An example of such a protein is the competitive inhibitor of the enzyme dihydrofolate reductase, which can be used in cancer treatment. These inhibitors mimic the substrate's shape, allowing them to fit into the active site and inhibit the enzyme's activity.


If you have isolated a bacterium that grows in a medium containing an organic substrate and nitrate in the absence of oxygen The nitrate is reduced to nitrogen gas This bacterium is?

This bacterium is likely a denitrifying bacterium. Denitrifying bacteria are able to use nitrate as a terminal electron acceptor in the absence of oxygen, converting it to nitrogen gas through denitrification.


When bacteria are present in sufficient numbers in the urine nitrate will be reduced to nitrite by the bacterial metabolism?

When bacteria are present in the urine, particularly certain gram-negative bacteria like Escherichia coli, they can reduce nitrate to nitrite through their metabolic processes. This reduction occurs because these bacteria possess the enzyme nitrate reductase, which facilitates the conversion of nitrate (NO3-) to nitrite (NO2-). The presence of nitrite in the urine is often used as a diagnostic indicator of urinary tract infections (UTIs), as it suggests the presence of nitrate-reducing bacteria. Thus, detecting nitrite in urine can aid in diagnosing infections and guiding treatment.


Why is silver nitrate not used in electroplating silver?

Silver nitrate is a compound used for making silver salts, not for electroplating silver. In electroplating, a pure silver metal source is needed as the source of silver ions, not silver nitrate. This is because the ions in silver nitrate are not stable in solution for electroplating.


Is biliverdin reductase in the liver or spleen?

primarily the liver