Garlic (Allium sativum L.) and garlic extracts have therapeutical properties that stem from their sulfur-containing compounds, mainly allicin. The main objective of this work was to compare conventional and "premium" garlic extracts in terms of yield and quality, with the latter being obtained using supercritical carbon dioxide (SC-CO2) as the solvent. Yield ranged between 0.65 and 1.0% and increased with extraction pressure (150-400 bar) at a constant temperature of 50°C. Extraction temperature (35-60°C), on the other hand, had little effect at a constant pressure of 300 bar. Based on yield and quality considerations, the best extraction conditions using SC-CO2 were 35-50°C and 300-400 bar. A yield of 5.5% was obtained by conventional extraction using ethanol as the solvent, but ethanol appeared to be less selective for valuable components than SC-CO2. The use of fresh garlic resulted in extracts that more closely resembled commercial products, possibly because of thermal and oxidative degradation of valuable microconstituents during drying.
70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.
The ethanol extraction process is used in the production of essential oils to extract the aromatic compounds from plant materials. Ethanol is a solvent that helps to dissolve and extract the essential oils, resulting in a concentrated and pure form of the desired fragrance or flavor.
Chlorophyll and other pigments in the chloroplasts were dissolved in the ethanol during the extraction process, leading to the green color of the solution.
75% ethanol is commonly used in RNA extraction because it helps to wash the RNA pellet by removing salts and other contaminants, while also helping to maintain the integrity and stability of RNA molecules. The lower ethanol concentration reduces the risk of RNA degradation and allows for efficient RNA recovery during the extraction process.
Ethanol and isopropyl extraction methods are both used to extract compounds from plants, but they differ in their solvent properties and extraction efficiency. Ethanol is a polar solvent that can extract a wide range of compounds, including both polar and non-polar molecules. Isopropyl, on the other hand, is a less polar solvent that may not be as effective at extracting certain compounds. Additionally, ethanol is generally considered safer for consumption compared to isopropyl, which is toxic if ingested. Overall, the choice between ethanol and isopropyl extraction methods depends on the specific compounds being targeted and the desired end use of the extracted material.
No, ethanol and water are miscible. For an extraction, you want two solvents that are immiscible (mutually insoluble). Standard choices for the organic phase are ethyl acetate or dichloromethane. For the aqueous phase, use water, aqueous acid or aqueous base, depending on the compound you are trying to isolate.
70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.
Ethanol precipitates DNA during the extraction process because DNA is not soluble in ethanol. When ethanol is added to the DNA solution, the DNA molecules become less soluble and clump together, forming a visible precipitate that can be collected and separated from the rest of the solution.
Seventy percent ethanol is commonly used in RNA extraction to wash and remove salts and contaminants from the RNA sample. It helps to purify the RNA by precipitating it out of the solution while leaving behind impurities. Additionally, the 70% ethanol concentration helps minimize RNA degradation during the extraction process.
Isopropanol is more preferred than ethanol in DNA extraction, as isopropanol facilitates precipitation more better, as it possess very less i.e., 0.6 to 0.7 volumes of alcohol.
The ethanol extraction process is used in the production of essential oils to extract the aromatic compounds from plant materials. Ethanol is a solvent that helps to dissolve and extract the essential oils, resulting in a concentrated and pure form of the desired fragrance or flavor.
Chlorophyll and other pigments in the chloroplasts were dissolved in the ethanol during the extraction process, leading to the green color of the solution.
75% ethanol is commonly used in RNA extraction because it helps to wash the RNA pellet by removing salts and other contaminants, while also helping to maintain the integrity and stability of RNA molecules. The lower ethanol concentration reduces the risk of RNA degradation and allows for efficient RNA recovery during the extraction process.
Ethanol and isopropyl extraction methods are both used to extract compounds from plants, but they differ in their solvent properties and extraction efficiency. Ethanol is a polar solvent that can extract a wide range of compounds, including both polar and non-polar molecules. Isopropyl, on the other hand, is a less polar solvent that may not be as effective at extracting certain compounds. Additionally, ethanol is generally considered safer for consumption compared to isopropyl, which is toxic if ingested. Overall, the choice between ethanol and isopropyl extraction methods depends on the specific compounds being targeted and the desired end use of the extracted material.
Cold ethanol is used in DNA extraction because it helps to precipitate the DNA molecules out of the solution. When DNA is mixed with cold ethanol, the DNA molecules become less soluble and clump together, making it easier to separate them from other cellular components. This process allows for the isolation and purification of DNA for further analysis.
Protein precipitation using ethanol can help to concentrate proteins in biological samples by causing them to clump together and separate from the solution. This can increase the efficiency of protein extraction by making it easier to isolate and purify the proteins of interest.
Yes, caffeine is soluble in ethanol. Caffeine, being a polar molecule, can dissolve in polar solvents like ethanol, which facilitates its extraction in various applications. This solubility is one reason why caffeine can be effectively extracted from coffee and tea using alcoholic solvents.