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phenolphthalein

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12y ago

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Why glycerol is added in bromophenol blue?

Glycerol is added to bromophenol blue to increase the density of the solution, allowing it to stay in the wells of gels or other media during electrophoresis. This helps prevent the dye from diffusing too quickly and ensures a more precise tracking of the migration of nucleic acids or proteins. Additionally, glycerol can help stabilize the dye and improve its solubility in aqueous solutions.


What would happen if you added phenolphthalein to sodium hydroxide?

Phenolphthalein is a pH indicator that turns pink in basic solutions. When added to sodium hydroxide, which is a strong base, the phenolphthalein will turn pink to indicate that the solution is basic.


Is azo benzene more polar than methylene blue?

Yes it is!


Is the universal indicator or phenolphthalein is more accurate?

Universal indicator is generally considered more accurate than phenolphthalein because it provides a broader range of colors to indicate the pH of a solution across a wider pH range. Phenolphthalein is more limited in its pH range and color changes.


What would happen if I placed a Vial of phenolphthalein into a jar with ammonia?

Nothing will happen... but when you put a drop of the vial in the phenolphthalein, the phenolphthalein will turn into pink... It was just an observation that I saw in our experiment this afternoon


Which dye would be more soluble in n-propyl alcohol the blue of the yellow?

Paper chromatography separates compounds based on their. A polar molecule has one end that is more positively charged than the other end. Blue dye is more polar compared to red.


When phenolphthalein is added to ammonium hydroxide is the color of the final solution is pink?

Phenolphthalein is an indicator often used in chemical titrations. Phenolphthalein is colorless in acidic solutions and turns pink in basic solutions. The more basic the solution the pinker the solution will become when Phenolphthalein is present.


Will phenolphthalein turn pink in coca-cola?

No, Coke would have to have a PH of more than 10 to turn pink. Coke only has a PH of 3


Function of bromophenolblue in agarose gel electrophoresis?

Bromophenol blue or commasive blue functions as a sample staining dye or DNA staining dye it is mixed with sample before loading the sample in wells. The migration of bromophenol blue is same as of DNA i.e. it carries negative charge and move in same direction of DNA with the speed equals to 200-400bp of DNA.It also prevent backflow of sample in vertical gel electrophoresis as the sample is light from the loading buffer which tends to come back from the well so bromophenol blue prevent the back flow.IUPAC NAME:2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-3-benzooxathiolyl]phenol.Bromphenol blue does not stain DNA. It is simply a dye that 1) helps you visualise your sample as you load it and 2) migrates (unrelated to the DNA) at a speed that is indeed equivalent to about 200-400bp of DNA, depending on the percentage of gel, giving an indication of how far your samples have run. It also does not prevent "backflow". Usually the buffer which you add to your DNA sample before loading on a gel (ie loading buffer) contains a dye such as bromophenol blue (there are others) and will also contain a dense substance, usually glycerol or ficoll. It is the glycerol or ficoll which due to its density will make the sample more dense than the buffer which the gel is run in, and will prevent it floating out of the well.In order to visualise (stain) the DNA you need an agent such as ethidium bromide or sybr green that intercalates with the DNA (slides between the basepairs) and fluoresces under UV light.Coommassie (not commasive) blue is a dye that will stain proteins (not DNA) but is used after the gel has been run to stain the gel. If you use it with an agarose gel, I'm guessing - having never tried it) you would just simply make a big blue mess and not see anything.


Does it take more NaOH in back titration with phenolphthalein?

Yes, it takes more NaOH in a back titration with phenolphthalein compared to a direct titration because the indicator reacts with the excess acid in the sample before the endpoint is reached. This means more base is required to neutralize the excess acid present.


Are polar bears the heaviest animal and why?

No polar bears are not the heaviest animal ad sperm or blue whale is. The reason why is because whales live much longer and they need more blubber on there long body.


Which is more polar acetonitrile or methnol?

Which is more polar acetonitrile or methnol?