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Answer- Flames in atomic emission are more sensitive to flame instability because optimum excitation conditions vary widely from element to element. High temperatures are needed for excitation of some elements and low temperatures for others. The region of flame that gives rise to optimum line intensities varies from element to element. Flame is rarely use in atomic emission because atomization is more complete when using a plasma due to the production of high temperatures. Also the plasma helps reduce the ionization interference effects. In flame absorption, after the sample is nebulized by a flow of gaseous oxidant, mixed with a gaseous fuel and carried into the flame it is then atomized. Then some of the atoms in the gas ionize to form cations and electrons. In flame emission the sample is introduce with argon, carries the sample into the flame. The flame is suppose to atomize the sample, while the flow of gas takes the ions and electrons to be detected.
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What is difference between an HPLC UV detector and a fluorescence detector?
HPLC UV detectors measure absorbance of UV light at a specific wavelength, while fluorescence detectors measure the emission of light at a longer wavelength after excitation with UV light. Fluorescence detectors are more sensitive and selective than UV detectors, but may require additional steps such as derivatization for certain compounds.
What are the differences between UV-Vis and IR spectroscopy techniques in terms of their applications and capabilities?
UV-Vis spectroscopy is used to analyze the absorption of ultraviolet and visible light by molecules, providing information on electronic transitions. It is commonly used for analyzing organic compounds and determining their concentration. In contrast, IR spectroscopy measures the absorption of infrared light, providing information on molecular vibrations. This technique is useful for identifying functional groups in organic compounds and determining molecular structure. Overall, UV-Vis spectroscopy is more sensitive to electronic transitions, while IR spectroscopy is more sensitive to molecular vibrations.
What is fluorescence in diamonds?
Fluorescence in diamonds is a natural phenomenon where the diamond emits visible light when exposed to ultraviolet light. This causes the diamond to glow in different colors, such as blue or green. The presence of fluorescence can affect the diamond's appearance and value, depending on the intensity and color of the fluorescence.
What are the differences between rhodamine 640 and rhodamine 6G?
Rhodamine 640 is a red-emitting dye while Rhodamine 6G is a green-emitting dye. Rhodamine 6G has a higher fluorescence quantum yield and is more photo-stable compared to Rhodamine 640. Additionally, their absorption and emission wavelengths differ, with Rhodamine 640 having higher absorption and emission wavelengths than Rhodamine 6G.
Does a diamond have fluorescence?
Every individual diamond may or may not have a natural fluorescence. This characteristic is both a scientific factor and an emotional -- eye candy -- factor in a diamond. You can read more, below.
What is the significance of the GFP extinction coefficient in determining the efficiency of fluorescence measurements?
The GFP extinction coefficient is important in determining how efficiently a substance absorbs light and emits fluorescence. A higher extinction coefficient means better absorption of light, leading to more accurate and sensitive fluorescence measurements.
When is atomic absorption more sensitive than atomic emission in atomic absorption spectrometers?
Atomic absorption is more sensitive to atomic emission when the excitation potential is greater than 3.5eV.
What are the strenghts of atomic absorption spectrometry compared to atomic emission spectrometry?
Atomic absorption spectrometry is more sensitive than atomic emission spectrometry.
What is difference between an HPLC UV detector and a fluorescence detector?
HPLC UV detectors measure absorbance of UV light at a specific wavelength, while fluorescence detectors measure the emission of light at a longer wavelength after excitation with UV light. Fluorescence detectors are more sensitive and selective than UV detectors, but may require additional steps such as derivatization for certain compounds.
Why spectrofluorometry potentially more sensitive than spectrophotometry?
Due to luminescence (i.e fluorescing capability of molecule), which is inherently more sensitive then absorption. Imagine dark stadium, 1 flashlight turns on. You can see it. That's luminescence. Now imagine the stadium with 500 flashlights on and 1 gets turned on. You won't really notice, i.e. less sensitive, like absorption. In luminescence and spectrofluorometry the background is dark with all the other non-fluorescing molecules. The ones the fluoresce are clearly seen and can be detected to individual molecules.
What are the differences between UV-Vis and IR spectroscopy techniques in terms of their applications and capabilities?
UV-Vis spectroscopy is used to analyze the absorption of ultraviolet and visible light by molecules, providing information on electronic transitions. It is commonly used for analyzing organic compounds and determining their concentration. In contrast, IR spectroscopy measures the absorption of infrared light, providing information on molecular vibrations. This technique is useful for identifying functional groups in organic compounds and determining molecular structure. Overall, UV-Vis spectroscopy is more sensitive to electronic transitions, while IR spectroscopy is more sensitive to molecular vibrations.
Is the computer light a natural light?
No. The computer light is not a natural light. Computer lighting contains certain chemicals that produce light due to a previous absorption of electromagnetic radiation of a different wavelength. This is known as fluorescence (like your fluorescence lamps). However, to ensure a longer lasting light supply, phosphorescent materials are sourced that are more efficient that fluorescence, and the method of cathodoluminescence, involving cathode rays is then applied on materials before being used as part of a monitor screen.
What is pulse flow?
Pulse flow is a type of irrigation technique where water is delivered in periodic pulses or bursts rather than continuously. This method helps in better water distribution and absorption by the plants, resulting in more efficient water usage and reduced water wastage. It is especially beneficial for watering sensitive crops and in areas with water scarcity.
What is the comparative and superlative of the word sensitive?
Comparative: more sensitive Superlative: most sensitive
What is fluorescence in diamonds?
Fluorescence in diamonds is a natural phenomenon where the diamond emits visible light when exposed to ultraviolet light. This causes the diamond to glow in different colors, such as blue or green. The presence of fluorescence can affect the diamond's appearance and value, depending on the intensity and color of the fluorescence.
Advantages and disadvantages of folin-lowry assay?
advantages: although it requires no digestion, it is very sensitive. it is 10 to 20 times more sensitive than ultraviolet absorption at 289nm and it is simple Disadvantages: the amount of colour varies with different proteins, the colour is not strictly proportional to the concentration
What are the differences between rhodamine 640 and rhodamine 6G?
Rhodamine 640 is a red-emitting dye while Rhodamine 6G is a green-emitting dye. Rhodamine 6G has a higher fluorescence quantum yield and is more photo-stable compared to Rhodamine 640. Additionally, their absorption and emission wavelengths differ, with Rhodamine 640 having higher absorption and emission wavelengths than Rhodamine 6G.
