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advantages: although it requires no digestion, it is very sensitive. it is 10 to 20 times more sensitive than ultraviolet absorption at 289nm and it is simple
Disadvantages: the amount of colour varies with different proteins, the colour is not strictly proportional to the concentration
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What are the advantages and disadvantages of Bradford protein assay?
Advantages: Compatible with most buffers, reagents and preparations. One step procedure that is sensitive and accurate. Disadvantages: Non linear correlation over large range of protein concentrations. The dye binds to surface of cuvettes so calibration curves need to be made every time. Also the dye binds to test tubes so washing after use immediate use is a must. High concentration detergents can interfere with assay.
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