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The Iodine test uses Iodine solution, which is usually a brown colour. It is used to test for the presence of Starch. Iodine solution, iodine dissolved in an aqueous solution of potassium iodide, reacts with starch producing a blue black color. If the sample being tested doesn't turn blue-black, then starch isn't present.

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Why do you need to add iodine solution to a specimen?

Iodine solution is commonly used to test for starch. If starch is present in a substance (e.g. a leaf which undergone photosynthesis) then the iodien solution would turn blue black. If no starch is present then it remains as light brown.


Why staining with iodine white cloth was brown while light green coloured shirt was blue black?

Starch would have been present on the light green shirt that reacted with the iodine forming a blue black colour.


What is the function of iodine?

Iodine solution is commonly used to test for starch. If starch is present in a substance (e.g. a leaf which undergone photosynthesis) then the iodien solution would turn blue black. If no starch is present then it remains as light brown.


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Iodine forms a complex with starch molecules when they are present together. This complex results in a change in the electronic structure of iodine, causing it to absorb light differently and exhibit a blue-black color, contrasting with its original brown hue.


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