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Bacteria in the milk ferment the lactose (milk sugar) into lactic acid, which has a sour taste. It also coagulates the casein (a protein) in the milk, causing it to separate into a thin watery layer (whey) and a thick clumpy substance. Cooling the milk slows the growth of bacteria; also, commercial milk is pasteurized, which kills most of the bacteria. Together, this is what keeps milk in the refrigerator from immediately going sour.

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Q: Why milk turn sour after left overnight?
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