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Grinding or heating can damage and inactivate sub cellular fractions when homogenizing.

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Q: Why must homogenizing buffer be kept ice cold?
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In cell isolation why must cold buffer and centrifugation be carried in cold temperatures?

TO STOP ENZYME ACTIVITY


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Why must the diazonium salt be kept cold?

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Blood kept at 4 degree temperature must not be traansfused to a patient immediately why?

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