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In High-Performance Liquid Chromatography (HP-LC), the detector functions to identify and quantify the separated analytes as they elute from the chromatographic column. It converts the chemical signals from the eluting compounds into measurable signals, typically in the form of electrical responses. Common types of detectors include UV-Vis, fluorescence, and mass spectrometry, each offering different sensitivity and selectivity based on the properties of the analytes. The resulting data is then used to create a chromatogram, which helps in analyzing the composition of the sample.
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What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
What is the function of diode detector?
the function of a diode detector is to detect stuff
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
How does an RID detector work for HPLC?
An RID (Refractive Index Detector) for HPLC works by measuring changes in refractive index caused by the presence of analytes eluting from the column. As analytes pass through the detector cell, they displace the mobile phase, causing changes in refractive index that are detected and converted into a signal. The signal is then plotted against retention time to create a chromatogram that can be used to identify and quantify analytes in the sample.
Definition of standard in HPLC?
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
What is post run in HPLC?
Post run in HPLC refers to the time after the completion of a chromatographic analysis where the system continues running to ensure that any remaining compounds are fully flushed out of the column and detector to prevent contamination and achieve a clean baseline for subsequent runs. It is an important step to maintain the integrity and performance of the HPLC system.
What is the function of detector in UV spectrophotometer?
Detector converts optical signal to electric signal
Which is more sensitive dector in hplc VWD or PDA?
In HPLC, a Photodiode Array (PDA) detector is generally more sensitive than a Variable Wavelength Detector (VWD). This is because the PDA can simultaneously measure absorbance across multiple wavelengths, allowing for better detection of compounds with varying absorbance characteristics. Additionally, the ability to collect spectral data enhances the identification and quantification of analytes, contributing to improved sensitivity.
