MW of a double-stranded DNA molecule = (# of base pairs) X (650 daltons/base pair)
Average weight of a DNA basepair (sodium salt) = 650 daltons
Cytosine, a pyrimidine (sugar) base, pairs with Guanine, a purine (nitrogen) base.
Base pairs in DNA molecules are held together by hydrogen bonds between the nitrogenous bases.
During transcription, the hydrogen bonds between the complementary base pairs of DNA (adenine-thymine and guanine-cytosine) are broken to allow the RNA polymerase enzyme to create a single-stranded messenger RNA (mRNA) copy of the gene.
GC base pairs are more stable than AT base pairs because they have three hydrogen bonds holding them together, while AT base pairs have only two hydrogen bonds. This extra bond in GC pairs makes them stronger and more difficult to break apart.
In biotechnology, base pairs refer to the complementary pairing of nitrogenous bases in DNA molecules. Adenine pairs with thymine, and guanine pairs with cytosine. Understanding base pairs is crucial for techniques like PCR and DNA sequencing.
It breaks the hydrogen bonds between the base pairs
In DNA, complementary base pairing occurs between adenine (A) and thymine (T), as well as cytosine (C) and guanine (G). These base pairs form the double helix structure of DNA through hydrogen bonding.
four base pairs
Uracil is the base in RNA that pairs with adenine.
with hydrogen bonds between base pairs 2 between A and T and 3 between C and G
Okazaki fragments are typically around 100-200 base pairs long in prokaryotes and around 1000-2000 base pairs long in eukaryotes.
Chargaff's rule states that hydrogen bonds can form only between certain complementary base pairs in DNA. adenine pairs with thymine (or uracil in RNA) with two hydrogen bonds, while guanine pairs with cytosine with three hydrogen bonds.