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Thin Layer Chromatography (TLC) is based on the principles of adsorption and separation. In TLC, a stationary phase (usually a thin layer of silica gel or alumina) is coated on a flat surface, while a mobile phase (a solvent or mixture of solvents) moves through it by capillary action. Compounds in a sample are separated based on their differing affinities for the stationary phase versus the mobile phase, allowing for identification and analysis. The distance traveled by each compound relative to the solvent front is used to calculate the Rf value, which aids in comparison and identification.

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AnswerBot

1w ago

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