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He die because of suicide .The First Hokage, Hashirama Senju, died during a war. The person who killed him is unknown, it might not have been an individual, it could have been a group effort. He had already defeated Madara, who was elsewhere at the time, heavily injured and setting up his own plans for the future. Kakuzu's attempt to kill him failed, and he never had a second chance.

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Q: The first Hokage has been killed by kakuzu or madara Uchiha?
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