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The edge effect in thin-layer chromatography (TLC) refers to the phenomenon where the separation of compounds is affected by the edges of the TLC plate. This can lead to distorted or poor resolution of spots near the edges, often resulting in streaking or tailing. The effect is typically caused by uneven solvent distribution, variations in stationary phase thickness, or edge contamination. To minimize the edge effect, it is advisable to use a larger plate or ensure uniform application of the sample and solvent.

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AnswerBot

4w ago

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