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Increasing the column length generally enhances the separation efficiency of a chromatography column, allowing for better resolution of compounds. However, it can also lead to a decrease in column capacity, as longer columns may result in greater back pressure and longer analysis times. Additionally, the increased length may require more mobile phase to achieve the same flow rate, potentially affecting the overall capacity of the system. Thus, while longer columns can improve separation, they may also complicate capacity and operational efficiency.

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