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10ml of 0.4M Citric acis solution
90 ml of 0.4M sodium phsophate dibasic solution
12.22g NaCl (tomake 150mM ionic solution) or 6.38g to make 100mM ionic strength.
make up volume upto 2000ml with water.
You should not need to pH this buffer . :)
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How do you calculate the atomicity of ammonium phosphate?
20.
How do you prepare 100mM ammonium acetate?
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
How long does it take codeine phosphate to work?
20 minutes to an hour
Where the single super phosphate use?
single super phosphate or superphosphate of lime, Ca(H 2 PO 4 ) 2 , is a compound produced by treating rock phosphate with sulfuric acid or phosphoric acid, or a mixture of the two. It is the principal carrier of phosphate, the form of phosphorus usable by plants, and is one of the world's most important fertilizers. Ordinary superphosphate contains about 20% available phosphate; double superphosphate (also called treble superphosphate) contains 40%-50% available phosphate.
How many ions are at Sodium Phosphate?
The chemical formula for Sodium Phosphate is Na3PO4.Therefor there are 8 atoms in one molecule of Sodium Phosphate; Three Sodium atoms, One Phosphorus atom, and Four Oxygen atoms. Sodium Phosphate is also known as Trisodium phosphate or TSP.
How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?
Without phosphate you can't.
I have to prepare a 1 to 10 dilution and have a 2ml culture suspension and want to prepare a 1 to 10 dilution of the entire culture.?
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
How do you make 0.2M citrate buffers for pH 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 and 7?
------Headings-------- pH Total conc. (M) Conjugate Base Conc. Conjugate Acid Conc. Conjugate Base Conjugate Acid -------------------------- 1.0 0.2 0.00141 0.19859 Dihydrogen Citrate Citric Acid 1.5 0.2 0.00438 0.19562 Dihydrogen Citrate Citric Acid 2.0 0.2 0.01322 0.18678 Dihydrogen Citrate Citric Acid 2.5 0.2 0.03658 0.16342 Dihydrogen Citrate Citric Acid 3.0 0.2 0.08290 0.11710 Dihydrogen Citrate Citric Acid 3.5 0.2 0.13825 0.06175 Dihydrogen Citrate Citric Acid 4.0 0.2 0.02903 0.17097 Hydrogen Citrate Dihydrogen Citrate 4.5 0.2 0.06988 0.13012 Hydrogen Citrate Dihydrogen Citrate 5.0 0.2 0.12588 0.07412 Hydrogen Citrate Dihydrogen Citrate 5.5 0.2 0.16860 0.03140 Hydrogen Citrate Dihydrogen Citrate 6.0 0.2 0.05695 0.14305 Citrate Hydrogen Citrate 6.5 0.2 0.11146 0.08854 Citrate Hydrogen Citrate 7.0 0.2 0.15985 0.04015 Citrate Hydrogen Citrate Since citric acid has three pKa's (3.15, 4.77, 6.40), , the one closest to each desired pH should be used to make the buffer. For example, if a pH 4.9 buffer is desired, the second pKa should be used and the conjugate acid/base pair for this pKa is dihydrogen citrate and hydrogen citrate. Each of these conjugate acid/base concentrations can be multiplied by the overall volume desired to get the desired volume. However, if not all four compounds are readily available (which is quite common), each one can be generated with acid/base chemistry. For example, if only 1.0 M citric acid is available and 100 ml of 0.2 M pH 7.0 buffer is required, 0.2*0.100/1.0 = 0.02 L or 20 ml of citric acid should be used and (0.15985*3+0.04015*2) *0.100/1.0= 0.055985 L or 55.985 ml of 1.0 NaOH should be added to the citric acid to make the required amount of citrate and hydrogen citrate. This makes up for 75.985 ml and the rest 24.015 ml should be topped up with distilled water.
How do prepare 1X TE Buffer from 5X TE Buffer?
1 ml of 5X TE in 4ml distilled water (or).......if u want 100 ml just multiply 1 and 4 with 20....you will get 20 ml 5X TE in 80 ml distilled water
How do you prepare 20 mM tris hcl buffer pH 7.4?
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?
Molarity of 1X PBS is 10mM. To prepare 20mM PBS take 16g of NaCl, 0.4 g KCl, 2.88 g Na2HPO4 0.48 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter.
What is the difference between tbs buffer-tbst buffer and corresponding function of them?
Tween 20. In TBST you add 0.05-0.1/ Tween 20.
How do you calculate the atomicity of ammonium phosphate?
20.
How do you prepare 100mM ammonium acetate?
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
What are the ages required for being a buffer lass?
about 20 to 30 years old
What is obb buffer in mrna extracton?
The composition of Buffer OBB is:20 mM Tris·Cl, pH 7.51 M NaCl2 mM EDTA0.2% SDSANSWERED BY .........BASIM
How long does it take codeine phosphate to work?
20 minutes to an hour
