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How much elemental iron in 100 mg ferric ammonium citrate?
Ferric ammonium citrate contains about 20% elemental iron. So, in 100 mg of ferric ammonium citrate, there would be approximately 20 mg of elemental iron.
How do you calculate the atomicity of ammonium phosphate?
To calculate the atomicity of ammonium phosphate, first determine the formula of ammonium phosphate, NH4)3PO4. The atomicity is then determined by adding the total number of atoms in the formula, which is 12 (4 nitrogen atoms from ammonium and 8 atoms from phosphate). So, the atomicity of ammonium phosphate is 12.
How do you prepare 100mM ammonium acetate?
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
How many ions are at Sodium Phosphate?
The chemical formula for Sodium Phosphate is Na3PO4.Therefor there are 8 atoms in one molecule of Sodium Phosphate; Three Sodium atoms, One Phosphorus atom, and Four Oxygen atoms. Sodium Phosphate is also known as Trisodium phosphate or TSP.
Does DNA contain phosphate?
"DNA is essentially made up of a sequence of nucleotides, each of which are associated with one molecule of phosphate." This is true, however not completely. Let's look at an example. Say we have a DNA molecule that is 10 base pairs long ( double stranded, so actually has 20 bases). The statement suggests we would have 20 phosphates in this molecule of DNA. However, we actually have 24. This is because the nucleotides situated at the 5' terminals of each strand have 3 phosphates rather than one. Since we have 2 5' terminals we have an excess of 4 phosphates which we did not account earlier, so instead of 20, we are now at 24 phosphates.
How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?
To prepare 20 mM potassium dihydrogen phosphate buffer with only dihydrogen phosphate, you can dissolve an appropriate amount of potassium dihydrogen phosphate in water to achieve the desired concentration of 20 mM. Since you are starting with only dihydrogen phosphate, you would need to adjust the pH of the solution by adding a strong base like sodium hydroxide until the desired pH (usually around 6.8) is reached.
How do you make 0.2M citrate buffers for pH 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 and 7?
------Headings-------- pH Total conc. (M) Conjugate Base Conc. Conjugate Acid Conc. Conjugate Base Conjugate Acid -------------------------- 1.0 0.2 0.00141 0.19859 Dihydrogen Citrate Citric Acid 1.5 0.2 0.00438 0.19562 Dihydrogen Citrate Citric Acid 2.0 0.2 0.01322 0.18678 Dihydrogen Citrate Citric Acid 2.5 0.2 0.03658 0.16342 Dihydrogen Citrate Citric Acid 3.0 0.2 0.08290 0.11710 Dihydrogen Citrate Citric Acid 3.5 0.2 0.13825 0.06175 Dihydrogen Citrate Citric Acid 4.0 0.2 0.02903 0.17097 Hydrogen Citrate Dihydrogen Citrate 4.5 0.2 0.06988 0.13012 Hydrogen Citrate Dihydrogen Citrate 5.0 0.2 0.12588 0.07412 Hydrogen Citrate Dihydrogen Citrate 5.5 0.2 0.16860 0.03140 Hydrogen Citrate Dihydrogen Citrate 6.0 0.2 0.05695 0.14305 Citrate Hydrogen Citrate 6.5 0.2 0.11146 0.08854 Citrate Hydrogen Citrate 7.0 0.2 0.15985 0.04015 Citrate Hydrogen Citrate Since citric acid has three pKa's (3.15, 4.77, 6.40), , the one closest to each desired pH should be used to make the buffer. For example, if a pH 4.9 buffer is desired, the second pKa should be used and the conjugate acid/base pair for this pKa is dihydrogen citrate and hydrogen citrate. Each of these conjugate acid/base concentrations can be multiplied by the overall volume desired to get the desired volume. However, if not all four compounds are readily available (which is quite common), each one can be generated with acid/base chemistry. For example, if only 1.0 M citric acid is available and 100 ml of 0.2 M pH 7.0 buffer is required, 0.2*0.100/1.0 = 0.02 L or 20 ml of citric acid should be used and (0.15985*3+0.04015*2) *0.100/1.0= 0.055985 L or 55.985 ml of 1.0 NaOH should be added to the citric acid to make the required amount of citrate and hydrogen citrate. This makes up for 75.985 ml and the rest 24.015 ml should be topped up with distilled water.
How do prepare 1X TE Buffer from 5X TE Buffer?
To prepare 1X TE buffer from 5X TE buffer, you would dilute the 5X TE buffer by mixing 1 part of the 5X buffer with 4 parts of water. For example, mix 1 ml of 5X TE buffer with 4 ml of water to obtain 5 ml of 1X TE buffer.
I have to prepare a 1 to 10 dilution and have a 2ml culture suspension and want to prepare a 1 to 10 dilution of the entire culture.?
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?
1x PBS buffer typically has a molarity of around 0.01 M. To prepare a 20 mM PBS buffer, you would need to dilute the 1x PBS stock solution with water. For example, to make 1 liter of 20 mM PBS buffer, you would need to mix 2 ml of 1 M PBS stock solution with 98 ml of water.
What is the recipe for washing buffer?
The usual wash buffer is PBS Tween. Na2HPO4 10.9 g, NaH2PO4 3.2 g NaCl 90 g. Distilled water to 1 Liter Mix to dissolve pH should be close to 7.4. Add 5 ml of Tween 20. Store this solution at room temperature. Dilute 100ml of this with 900 ml of distilled water before use.
How do you prepare 20 mM tris hcl buffer pH 7.4?
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
How much elemental iron in 100 mg ferric ammonium citrate?
Ferric ammonium citrate contains about 20% elemental iron. So, in 100 mg of ferric ammonium citrate, there would be approximately 20 mg of elemental iron.
What is the difference between tbs buffer-tbst buffer and corresponding function of them?
Tween 20. In TBST you add 0.05-0.1/ Tween 20.
How do you prepare master mix?
To prepare a PCR master mix, combine all the components (such as buffer, dNTPs, primers, and DNA polymerase) in a tube according to the recipe. Mix them thoroughly by gentle pipetting and then aliquot the master mix into smaller tubes to avoid repeated freeze-thaw cycles. Store the aliquots at -20°C or -80°C until use.
How do you calculate the atomicity of ammonium phosphate?
To calculate the atomicity of ammonium phosphate, first determine the formula of ammonium phosphate, NH4)3PO4. The atomicity is then determined by adding the total number of atoms in the formula, which is 12 (4 nitrogen atoms from ammonium and 8 atoms from phosphate). So, the atomicity of ammonium phosphate is 12.
How much prime rib should I prepare for 20 adults?
For 20 adults, you should prepare around 10-12 pounds of prime rib.
