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Q: How methanol activates pvdf membrane?
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Why to immerse PVDF membrane in methanol?

We normally do that to activate the membrane.


Why acetone free methanol in lieshmans stain?

methanol for fixing the smear and acetone free to avoid cell membrane lysis


Is that 50 percent methanol damage membranes more or 25 percent acetone?

50% methanol will damage the membrane more than 25% acetone. we did the lab.


What is a calcium ion receptor?

This is a protein on or embedded in a cell membrane that activates when a Calcium ion interacts with it.


Effect of methanol on beet membrane?

Methanol breaks down the structure of the beet membrane which in turn release the pigment, betcyanin, the pigment found in beet cell vacuoles that give beets their characteristic colour, and releases it. The more intense the colour you see, the greater damage that had been done on the membrane.


Character of a Western blot?

Western blotting is a technique to detect specific proteins from a sample such as cell or tissue lysates. Western blot is a membrane (nitrocellulose or PVDF) on which the proteins are transferred for further analysis. Proteins on the blot are visualized by specific antibodies.


Explain the mechanism of action of nonsteroid hormones?

All non steroid hormones are proteinacious and can't enter cell .they attach with fixed membrane receptor which activates an enzymes that activates a second messenger i.e. cAMP that carries message in side cell .


What is a Large oval structure that directs all of the cells activates?

cell membrane


What is methanol's shape?

methanol is liquid


What compounds are found in methanol?

Methanol is an alcohol and consists of carbon,hydrogen and oxygen its formula is CH3OH


What is blotting and types of blotting?

Blotting is a technique of transferring DNA or RNA or Protein from a gel to the membrane. nitrocellulose, PVDF or nylon membrane are used to attach these molecules permanently on them. Western, southern and norther blotting are the techniques used to transfer protein, DNA and RNA respectively. It is done to detect them with a specific probe or antibody. This can not be detected or easy to handle on the gel, so we do blot for these analysis.


Is methanol a solid?

No, at room temperature methanol is a liquid.