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potassium acetate (KAc) is added, which does three things:

a. Circular DNA is allowed to renature. Sheared cellular DNA remains denatured as single stranded DNA (ssDNA).

b. The ssDNA is precipitated, since large ssDNA molecules are insoluble in high salt.

c. Adding sodium acetate to the SDS forms KDS, which is insoluble. This will allow for the easy removal of the SDS from your plasmid DNA.

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