The relative difference in velocity between two acid concentrations is independent of the
temperature.
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
its ok to use
you can prepare 20nM sodium phosphate buffer pH7.0. calculate mass of enzyme and dilute in volume buffer to reach concentration you want. good luck
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?
Disodium hydrogen phosphate dihydrate has the role of a buffer in some insulin receipts.
The concentration.
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
its ok to use
preparation of 5.8 ph phosphate buffer
you can prepare 20nM sodium phosphate buffer pH7.0. calculate mass of enzyme and dilute in volume buffer to reach concentration you want. good luck
By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer
5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).
concentration of buffer effects in buffer strength
Tris EDTA buffer inhibits nucleases that will degrade the DNA, by chelating cations required by the enzymes. Phosphate buffer offers no such protection against degradation.
bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.
The phosphate buffer system consists of two ions: dihydrogen phosphate ions and hydrogen phosphate ions. When the number of hydrogen ions in a body's bloodstream increases (pH drops), hydrogen phosphate ions accept hydrogen ions in order to maintain the equilibrium between the concentration of hydrogen and hydroxide ions within the bloodstream. When the number of hydrogen ions in the bloodstream decreases (pH increases), the resulting dihydrogen phosphate ions release hydrogen ions in order to increase the number of hydrogen ions in the blood.
1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system