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Copper sulphate when mixed with a protein will turn purple if the amount of protein is sufficient enough in quantity to trigger the reaction. Planex1234 says; No offense but i think it is starch that turns dark blue - purple. Thetada says: the colour changes are arguably similar. Iodine solution goes blue/black in the presence of starch. The colour that sodium hydroxide / copper sulphate solution goes in the presence of protein is variously described as mauve / purple.

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11y ago
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13y ago

It will turn a Purple shade . No heat needs to be applied so the result is direct. Eg. Sodium Hydroxide (Colourless/Clear) + Copper Sulfate (Blue) + Milk- Protein Source (White) = A solution of all three liquids (Purple)

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12y ago

bule or reddish orange

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Q: What is the change in color in testing for the presence of protein in egg albumin using solution sodium hydroxide and copper sulfate?
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Why protein solution turns purple when copper sulfate solution is added?

Copper sulfate solution provides cupric ions that react with the peptide bonds present in the protein molecule. The test is called as biuret test, as it was originally given by biuret compound.( when urea is heated it forms biuret). The reaction is due to the formation of complex between the cupric ions and lone pair of electrons present on the Nitrogen and Oxygen atoms of peptide bond.

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