Copper sulphate when mixed with a protein will turn purple if the amount of protein is sufficient enough in quantity to trigger the reaction. Planex1234 says; No offense but i think it is starch that turns dark blue - purple. Thetada says: the colour changes are arguably similar. Iodine solution goes blue/black in the presence of starch. The colour that sodium hydroxide / copper sulphate solution goes in the presence of protein is variously described as mauve / purple.
It will turn a Purple shade . No heat needs to be applied so the result is direct. Eg. Sodium Hydroxide (Colourless/Clear) + Copper Sulfate (Blue) + Milk- Protein Source (White) = A solution of all three liquids (Purple)
bule or reddish orange
its an enzyme enzymes are a type of protein
Egg Albumin, to be specific, has high concentrations of polar amino acids such as Glutamic Acid, Aspartic Acid, and Lysine (basic). It also has high concentration of Leucine (a non-polar amino acid) and small amounts of many others. The high concentration of polar amino acids makes the hydrophilic polar/charged molecules face the outside, toward the water containing solution. While the hydrophobic portions of the protein are "Shelled" inward away from the water.
Sodium hydroxide can dissolve grease, oils, fats and protein based deposits. It helps convert these substances into a form of soap (the saponification process) which can then be easily washed away.
The bacterial proteins will become radioactive
Copper sulfate solution provides cupric ions that react with the peptide bonds present in the protein molecule. The test is called as biuret test, as it was originally given by biuret compound.( when urea is heated it forms biuret). The reaction is due to the formation of complex between the cupric ions and lone pair of electrons present on the Nitrogen and Oxygen atoms of peptide bond.
The reagent turns pink due to the presence of broken amino acid chains of the albumin, a source of protein.
Albuminuria
egg albumin contains protein. when hydrochloric acid is added to egg albumin, it denatures the protein. in the lab - the observations were as follows: the solution which was initially gel- like, turned watery and transparent
non
It's due to denaturation of protein by heat,alkaline/acidic solution etc
Biuret solution
Heller's test is used to clinically detect the presence of albumin (protein) in urine.
Albumin and pepsin are both proteins, the test for proteins was positive.
This test is performed to test the presence of cysteine in the test solution (protein solution)
the protein albumin is very expensive,so if keeped in good condition ,you can use it the time you want.
WHEN TESTED ON MILK- When milk(acid) is added with dilute Sodium Hydroxide it is mixed into an alkaline solution ,the copper sulphate is applied because the copper ions forms a purple complex with the Nitrogen from the peptide chain from the milk.The colour changes from light yellow to light purple.This proves the presence of protein in milk.
can protein (albumin) diffuse out of a dialysis tube and why