PBS (phosphate buffer saline) is used to maintain osmolarity of cells i.e. maintaining an isotonic environment. it is used to maintain pH of proteins at which its native structure could be maintained.
as the buffer for experiment
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
I'm not sure, but I know it's in my "Gatorade Rain"
The compound with formula Na3PO4 is named "sodium phosphate", "trisodium phosphate", "sodium ortho-phosphate", or "trisodium ortho-phosphate".
potassium phosphite
Phosphate is made of phosphorous and oxygen. The formula for the phosphate ion is PO43-.
It is just Phosphate buffered saline.
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration
sterilized (either by filter or by heat) Phosphate buffered Saline solution used to wash cells or for other biological applications
Phosphate buffered saline is a buffer solution commonly used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, potassium chloride and potassium phosphate. The osmolarity and ion concentrations of the solutions match those of the human body.
These r cells whose plasma have been filtered after centrifugating, and PBS( phosphate buffered saline) has been used in rinsing d cell components left after removing the plasma
The main function of PBS (phosphate buffer saline) is to maintain the pH of the solution. by Himanshu Goel Jaypee University of Information Technology
Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
Fixation is performed to preserve cells in a sample and to and to make the cells permeable to reagents and probes, then samples can be stored for a certain period of time, while the cells remain intact. Generally the amount of time they can remain intact is unknown. Paraformaldehyde fixative is the chemical that you use, along with NaOH and phosphate buffered saline.
Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
Your mo
Calcium/phosphate balance.