The PBS solution is necessary to check the stability of materials that will be used for biological purposes.
to check the pH balance
its ok to use
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?
Disodium hydrogen phosphate dihydrate has the role of a buffer in some insulin receipts.
See the Related Links for "Columbia.edu: Phosphate buffer automatic calculator" to the bottom for the answer.
its ok to use
preparation of 5.8 ph phosphate buffer
By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer
Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html
5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).
Tris EDTA buffer inhibits nucleases that will degrade the DNA, by chelating cations required by the enzymes. Phosphate buffer offers no such protection against degradation.
bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system
Without phosphate you can't.
Bonds to the phosphate
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?