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The PBS solution is necessary to check the stability of materials that will be used for biological purposes.

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14y ago
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16y ago

to check the pH balance

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Q: Why use a phosphate buffer in the lab?
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Related questions

If use sodium phosphate buffer instead of potassium phosphate buffer?

its ok to use


How to prepare phosphate buffer pH 5.8?

preparation of 5.8 ph phosphate buffer


How you prepare 10 mM phosphate buffer from 0.5 M phosphate buffer?

By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer


Is there any calculation to make phosphate buffer at pH 7?

Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html


How I can prepare 5 mM phosphate buffer?

5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).


Why is isolated DNA suspended in TE buffer only instead of any buffer like phosphate buffer or so?

Tris EDTA buffer inhibits nucleases that will degrade the DNA, by chelating cations required by the enzymes. Phosphate buffer offers no such protection against degradation.


Which buffer system acts the fastest?

bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.


What is the difference between phosphate buffer and phosphate buffered saline?

actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place


What are different types of buffering system?

1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system


How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?

Without phosphate you can't.


What happens when you add NaCl salt in it's solid state to a phosphate buffer system?

Bonds to the phosphate


Why is the phosphate buffer made up by using the Henderson hasselbalch equation not the expected PH?

1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?