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It makes it easier to analyze the data sets from separate experiments if you normalize. The curves themselves should approximate each other regardless of the initial parameters. If you normalize first the % recovery (immobile/mobile estimates) can be taken right form the curve without post analysis. Tau should be approximately the same regardless of normalization.

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Q: When curve fitting frap analysis data should the initial post-bleach phase intensity be normalised to zero?
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Canadian 1978 Red River Indian Siver Dollar?

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