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Yes you can do it, if you do it right!
* Use a Congo Red solution of pH 6-7 and a 1.0 cm Cuvette. * Adjust the concentration that at Lambda(max) near 500nm becomes ~1.4 AU's. * Read the first Spectrum. * Use a very fine capillary (< 0.2mm) and add slowly HCl-Gas!! till you detect the smallest possible Change in color! * Read the second Spectrum. * Add a minimal amount of HCl-Gas!! And read the third Spectrum. * Repeat th last step above till the indicator becomes real blue. * If you overlay now all read Spectra you will see your Isosbestic/Isobestic Point! *
It is important to use HCl-Gas and not an aqueous solution, because the volume must be constant during the whole Experiment!! About Isosbestic/isobestic Points you may also read on: Related Link Nr. 1. below.
What else can I help you with?
What can be used in place of Congo red dye?
orange and purple will make red that can replace the congo red dye..
What is the reaction of milk with Congo red?
when congo red was mixed with milk it produced pink color solution.
What ingredients are Congo Red made up of?
Congo Red is a sodium salt that has the chemical formula C32H22N6Na2O6S2. At one time, Congo Red was used as a dye for clothing but has since been abandoned due to its toxicity.
What is a nickname for Congo?
red country
Is Congo red autoclavable?
Yes.
What is the color of Congo red in base solution?
changes to violet in basic solution
African grey congo parrots?
I own one actually. The difference between Congo’s and Timneh’s are that Congo’s have a red tail and are slightly bigger. I own a Congo.
What are the Christmas colors in Congo?
red and green
What is the reaction of bile to litmus paper phenolphthalein and Congo red?
There is no change in litmus paper, phenophthalein turns yellow, and congo red changes to orange..
What is the difference in appreance between boiled and unboiled yeast cells in Congo red solution?
since the unboiled yeast is alive it will not allow the congo red to be absorbed and the boiled yeast is dead so it doesnt have any way to fight of the solution(congo red)
What is the pKa value of Congo red?
We think it is about 4, based on the lab we just did, but sometimes we make mistakes!
What is a Congo red stain?
it is some one who is dumb.
