Even before the medical community had embraced the germ theory, two physicians had observed that the use of sloppy methods and contaminated instruments during surgery led to increased mortality of the surgical patient.
Aseptic.
The best advantage of aseptic techniques is that harmful microbes are not introduced. For example in the surgical field or determining what microbe has caused what disease. An disadvantage is the cost of specialized clothing, materials, and sterilized instruments.
Semmelweis's ideas weren't accepted right away because they challenged the prevailing scientific beliefs of the time that miasma, or "bad air," caused disease. His theory, that handwashing could prevent the spread of disease, was also seen as radical and faced resistance from the medical community. Additionally, Semmelweis struggled to provide a scientific explanation for his findings, which made them harder to accept.
He was the first physician to use disinfection in a hospital setting. He used "carbolic acid" solution to disinfect medical apparatus in the operating room and strict hand-washing techniques to ensure midwives did not transfer infection from one patient to another.
Ignaz Semmelweis was a Hungarian physician who is known for promoting the importance of handwashing in medical settings to prevent the spread of infections, particularly childbed fever. His work helped pave the way for the germ theory of disease and was instrumental in improving hygiene practices in healthcare.
Ignaz Semmelweis
Isolation is the use of physical separation and strict aseptic technique for a patient who either has a contagious disease or is immunocompromised.
Louis Pasteur is considered the early microbiologist who was most responsible for developing sterile laboratory techniques. His work on pasteurization and germ theory laid the foundation for modern microbiology practices.
Short answer is Koch's postulates. Long answer you can't ascribe any scientific data you may gather to a bunch of bacteria on a plate or in a culture, as you won't know which of the bacteria gave you the result you observed. So if you are trying to figure out which bacteria causes a disease, inoculating a mix of bacteria won't tell you which one causes the disease. Isolating each one using aseptic technique and then inoculating individually and finding the disease in one animal will tell you which bacteria caused to disease.
Louis Pasteur demonstrated that microorganisms, such as bacteria and viruses, can cause disease when they infect living organisms. His experiments helped establish the germ theory of disease, which revolutionized our understanding of how illnesses spread and laid the foundation for modern microbiology and immunology.
He is Edward Jenner . He demonstrated the first safe vaccination procedure to treat a smallpox disease. Btw. I'm Kazy Quijano Malazarte. visit me on facebook! :)) Dadzlil_me@yahoo.com
B. anthracis was the first bacterium conclusively demonstrated to cause disease, by Robert Koch in 1876