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to make 500ml of 1x TAE solution we have to take 5ml of 100x TAE solution. mix it in 495 ml of deionized water.

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13y ago

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The stocks are commonly labeled as X factors such as 10X, 5X, 100X etc. X-factor indicates that the solution is concentrated and must be diluted usually with water to 1X concentration for use. For eg: - A 100X concentrated solution should be diluted to 100 fold. to convert 1X to 10X take one ml of 1x buffer in a measuring cylinder and dilute it to make it 10 ml. its now 10x buffer.


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See the link below.


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How much 10x TAE buffer will it take to make 500mL of 1x TAE solution?

preparation of 500 ml 1x TAE buffer,50ml of 10x buffer add to 450ml DI.water. to calculate just use MV=MV so 500mL * 1x= 10x * V then solve for V. add the amount of DI water you need to get the volume you calculated above.