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Q: Why is it necessary to correct absorbance reading?
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What is the actual absorbancy if 2mL of a culture is diluted by adding 4mL of water and the absorbancy reading of the diluted culture is 0.059?

The actual absorbance of the undiluted culture can be calculated by multiplying the absorbance reading of the diluted culture by the dilution factor. In this case, the dilution factor is 2 (total volume after dilution divided by initial volume), so the actual absorbance is 0.059 * 2 = 0.118.


Is wavelength or absorbance the dependent variable?

"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.


Which of these are correct spelling nesessary necasary necessary necessary?

The correct spelling is "necessary."


Is 'am reading a dictionary' correct English?

I am reading a dictionary. Comprehensible but not correct.


What is the purpose of the blank?

Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.


Which is correct If you feel it necessary or If you seem it necessary?

Neither is correct. You say "If you feel it is necessary" or "If you feel that it is necessary."


Why would it not be correct to blank the spectrophotometer with deionized water?

It would not be correct to blank the spectrophotometer with deionized water because deionized water does not contain any solutes that would contribute to the absorbance reading. The purpose of blanking the spectrophotometer is to zero the instrument by measuring the absorbance of a solution that only contains the solvent used in the sample. Using deionized water would not effectively remove any background interference from the sample, leading to inaccurate results. It is recommended to use a blank solution that closely matches the solvent and conditions of the sample being measured.


What is the Correct spelling of necessary?

The correct spelling of necessary is necessary. Very simple. ( Actually it's in the question.)


What is absorbance vs concentration?

Absorbance is a measure of the amount of light absorbed by a sample at a specific wavelength, typically measured using a spectrophotometer. Concentration is the amount of a substance present in a unit volume of a solution, often expressed in moles per liter (M). The relationship between absorbance and concentration is governed by Beer's Law, which states that absorbance is directly proportional to concentration and path length.


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What is difference between specific absorbance and absorbance interms of spectroscopy?

specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com


Why is the wet bulb reading necessary when checking the systems charge?

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