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What happens after genetically modified plasmids inserted into bacteria?
Plasmids are cloned and start producing protein .
What is genetic engineering in animals?
GE is removing DNA from selected animals then adding new genes (which posses different traits) into the DNA itself before implanting the cell into the host
What is a vector used for in a genetic engineering experiment?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
What is the methodology for producing recombinant DNA to be used in gene cloning?
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
What are the steps of using recombinant DNA technology?
1 Isolate DNA 2 Cut DNA with a restriction enzyme 3 Mix the DNA's and join then together by using DNA ligase 4 Insert the recombinant plasmid into a host bacterium 5 Allow the bacterium to reproduce
What is the viral DNA that is embedded in a host's DNA?
The viral DNA that is embedded in a host's DNA is called a provirus.
What are the steps in cloning DNA?
Isolate the DNA sequence to be cloned. Insert the DNA into a vector. Introduce the vector into a host organism. Allow the host organism to replicate the DNA. Isolate the cloned DNA from the host organism for further study or manipulation.
Why do viruses depend in host cells?
Viruses are composed of protein and DNA. The DNA encodes the protein as well as the DNA for the virus. Viruses depend on host cells because they are incapable of reproducing themselves. They enter the host cell and the viral DNA is inserted into the host DNA. The virus then "hijacks" the host cells replication machinery to make more viral protein and viral DNA.
What enzyme inserts viral DNA into the host's chromosomal DNA?
The enzyme responsible for inserting viral DNA into the host's chromosomal DNA is called integrase. Integrase plays a crucial role in the integration of the viral genome into the host cell's DNA, allowing the virus to replicate and persist within the host cell.
Does a lysogenic virus inject their DNA into the host cell?
No, a lysogenic virus integrates its DNA into the host cell's genome instead of injecting it. This integrated viral DNA, called a prophage, replicates along with the host cell's DNA.
What are the 3 stes of lysogenic infection?
The three stages of lysogenic infection are attachment, insertion (integration) of viral DNA into host DNA, and replication of the viral DNA along with host DNA.
What is the viral DNA that has attached to a host cell's chromosomes and that is replicated with the chromosome's DNA?
The viral DNA that has integrated into a host cell's chromosome is called a provirus or prophage. This integrated viral DNA is replicated along with the host cell's DNA during cell division, allowing the virus to be passed on to daughter cells.
What is the Significance of host cell in recombinant DNA technology?
The host cell is important in recombinant DNA technology because it is the organism that will replicate the recombinant DNA construct. The DNA construct is inserted into the host cell, which then uses its machinery to produce the desired protein or molecule encoded by the inserted DNA. The choice of host cell is critical as it can affect the efficiency of DNA replication, protein production, and post-translational modifications.
What engineering involves inserting foreign dna into host dna to make recombinant DNA?
genetic
The viral reproductive cycle in which a phage injects its DNA into a host cell and the DNA is inserted into the host cell's chromosome is called the?
lysogenic
How is an extracted DNA inserted into a new molecule?
Extracted DNA can be inserted into a new molecule through a process called molecular cloning. In this process, the DNA of interest is first integrated into a vector, which is a small DNA molecule capable of replicating inside the host cell. The new DNA construct can then be introduced into a host cell, where it will be replicated along with the host DNA.
An infection in which DNA of a virus is embedded into a host cell and replicates with host DNA is called what?
This process is called lysogeny, and the host cell is referred to as a lysogenic cell. The integrated viral DNA is known as a prophage.
