Aequorea GFP is a protein of 238 amino acids with a molecular weight of 27 or 30 kDa.
Source: http://www.cryst.bbk.ac.uk/PPS2/projects/jonda/intro.htm
In the context of watts, "mW" and "MW" represent different values. "mW" stands for milliwatts, which is one-thousandth of a watt, while "MW" stands for megawatts, which is one million watts. Therefore, a megawatt (MW) is significantly larger and more powerful than a milliwatt (mW) in terms of voltage.
1.493 mw
10.
1 MW is 1000 kW therefore 10 MW is equal to 10,000 kW.
1000
GFP tailswap refers to the swapping of the C-terminal sequence of a GFP (Green Fluorescent Protein) molecule with another protein or peptide sequence of interest. This modification allows the GFP to function as a fusion tag with the added sequence for specific experimental purposes, such as tracking the localization of the target protein in live cells.
No.
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
In the context of watts, "mW" and "MW" represent different values. "mW" stands for milliwatts, which is one-thousandth of a watt, while "MW" stands for megawatts, which is one million watts. Therefore, a megawatt (MW) is significantly larger and more powerful than a milliwatt (mW) in terms of voltage.
MW is the abbreviation for megawatts. mW is the abbreviation for milliwatts.
Inside bacterial cells, the green fluorescent protein (GFP) is typically encoded by a gene that can be introduced into the bacterial genome or expressed on a plasmid. The gene consists of coding sequences that allow the production of the GFP protein, which fluoresces green when exposed to specific wavelengths of light. The gene is regulated by bacterial promoters and terminators to control its expression level. The GFP protein is then synthesized within the bacterial cell and can be visualized using fluorescence microscopy or other techniques.
100 mW to W
1 kw = 0.001 mw 25 kw = 0.025 mw
1. Tamil Nadu (4906.74 MW)2. Maharashtra (2077.70 MW)3. Gujarat (1863.64 MW)4. Karnataka (1472.75 MW)5. Rajasthan (1088.37 MW)
The three-dimensional structure of green fluorescent protein (GFP) was determined using X-ray crystallography. Researchers crystallized GFP and then diffracted X-rays through the crystal to collect data on how the X-rays scattered. By analyzing the resulting diffraction patterns, they could reconstruct the electron density map of the protein, ultimately allowing them to model the three-dimensional structure. This method provided detailed insights into GFP's unique chromophore and its fluorescent properties.
1. Tamil Nadu (4906.74 MW)2. Maharashtra (2077.70 MW)3. Gujarat (1863.64 MW)4. Karnataka (1472.75 MW)5. Rajasthan (1088.37 MW)
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.