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It is important because the fragments will bond to other fragments with complementary single-stranded ends.

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What does the formation of recombinant DNA result from?

Recombinant DNATo to make recombinant DNA or plasmids, the two different samples of DNA need to be cut up by the same restriction enzyme. Restriction enzymes cut DNA at specific sequences (restriction sites) and is usually a staggered cut. For example, say you had the following sequence of DNA (both strands): 5' GAATTC 3'3' CTTAAG 5'Say the restriction enzyme used will cut a strand between a guanine and adenine on one strand and an adenine and guanine one the other strand. For the given DNA, there would be cuts where the bars are:5' G|AATTC 3'3' CTTAA|G 5'Then the strands would separate:5' G--------AATTC 3'3' CTTAA--------G 5'Because the cuts are staggered, hydrogen bonds are left open. The ends of the restriction fragments are called "sticky ends" because of their ability to bond to other fragments. Remember that both sets of DNA are cut with the same restriction enzyme. Therefore, the sticky ends of the restriction fragments are complementary to each other. Then you're able to take one fragment of one DNA sample and insert it into the other DNA sample, which are bound together by hydrogen bonds. DNA ligase is then added to seal the ends together.


What does the term sticky ends refer to in gene splicing?

Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.


What is a sentence with the word staggered?

I bumped my forehead on the wall and staggered to get to my room.


When did Staggered Crossing end?

Staggered Crossing ended in 2007.


When was Staggered Crossing created?

Staggered Crossing was created in 1997.


How do you use the word stagger in a sentence?

The drunk staggered out of the bar. He was staggered by the revelations about his uncle. Roadblocks set up at staggered intervals help to prevent car bombings.


What is a good sentence using staggered?

The wounded deer staggered on the path.


What is a sentence staggered?

This biplane only looks odd because of the staggered arrangement of wings. The heavy load staggered the poor donkey, but he carried on famously.


What is adverb to go with staggered?

Sacredly, Hurriedly , awkwardly , horribly, painfully, quietly, forcefully staggered.


What is a synonym of staggered?

stagger


The role of restriction enzymes in DNA technology is to?

Produce a cut (usually staggered) at a specific recognition sequences on DNA.


What are the ratings and certificates for Staggered - 1994?

Staggered - 1994 is rated/received certificates of: Germany:12 UK:15