answersLogoWhite

0

Recombinant DNA

To to make recombinant DNA or plasmids, the two different samples of DNA need to be cut up by the same restriction enzyme. Restriction enzymes cut DNA at specific sequences (restriction sites) and is usually a staggered cut. For example, say you had the following sequence of DNA (both strands): 5' GAATTC 3'

3' CTTAAG 5'

Say the restriction enzyme used will cut a strand between a guanine and adenine on one strand and an adenine and guanine one the other strand. For the given DNA, there would be cuts where the bars are:

5' G|AATTC 3'

3' CTTAA|G 5'

Then the strands would separate:

5' G--------AATTC 3'

3' CTTAA--------G 5'

Because the cuts are staggered, hydrogen bonds are left open. The ends of the restriction fragments are called "sticky ends" because of their ability to bond to other fragments. Remember that both sets of DNA are cut with the same restriction enzyme. Therefore, the sticky ends of the restriction fragments are complementary to each other. Then you're able to take one fragment of one DNA sample and insert it into the other DNA sample, which are bound together by hydrogen bonds. DNA ligase is then added to seal the ends together.

User Avatar

Wiki User

15y ago

What else can I help you with?

Related Questions

Types of DNA that result when genes from one organism are put into another organism?

recombinant DNA


DNA molecules that are produced by combining DNA from different sources are called?

The word you're looking for may be "recombinant".


What is a DNA molecule containing regions from different sources?

A DNA molecule containing regions from different sources is called recombinant DNA. This is often created in laboratories by combining DNA from different organisms or through genetic engineering techniques. Recombinant DNA technology has many applications in biotechnology and genetic research.


At what stage of meiosis recombinant DNA is made?

Recombinant DNA is made during the crossing over stage of meiosis, specifically during prophase I. This is when homologous chromosomes exchange genetic material, leading to the formation of new combinations of genes.


Splicing DNA from two different organisms produces a new DNA segment called?

When DNA contains parts from two or more organisms it is recombined. Recombinant DNA is often used in genetic engineering. A natural process of DNA recombination is called sexual reproduction.


An enzyme called what is added to attach the DNA backbone between vector and foreign DNA that have formed base pairs at the sticky ends during the formation of recombinant dna?

D ligase


What is the different between recombinant DNA and non-recombinant DNA?

Recombinant DNA is created by combining DNA from different sources, such as different species, through techniques like genetic engineering. Non-recombinant DNA refers to DNA that has not been modified in this way and only contains genetic material naturally found in an organism.


Why is recombinant DNA a new form of DNA?

Recombinant DNA is a new form of DNA because it is created via introduction of the relevant DNA into the existing organismal DNA.


Does recombinant DNA have something to do with plants?

Recombinant DNA is made of DNA taken from two different organisms. It may contain plant DNA but this is not always the case.


What seals the sticky ends of restriction fragments to make recombinant DNA?

These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.


What is it called when you insert DNA in to a bacterium?

Recombinant DNA


What is last step in the production of a recombinant DNA plasmid?

The last step in the production of a recombinant DNA plasmid is joining the DNA. This is done by adding DNA ligase to joint DNA fragments.