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Is cell a countable or uncountable noun Do I say 1 gram of cell or 1 gram of cells?
Wiki User
∙ 11y ago
The noun cell is a countable noun. You should say, "one gram of cells".
Wiki User
∙ 11y ago
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Who is the secretary of gram panchayat?
Gram sevak
What is the definition of gram?
1 gram is the weight of 1 cubic centimeter of pure water. 1 gram is one thousandth = 0.001 of a kilogram.
What does the suffix -gram mean?
The suffix gram means to draw or write. This is used in telegram.
What is the abbreviation for grams?
The abbreviation for gram is gm. and the symbol is g. Gram is short g.
What does the greek prefix gram mean?
The prefix gram means to write or draw. An example is gramophone.
Can you gram stain a cell without a cell wall?
No bacteria exist without a cell wall! If they loose cell wall then they die. Gram staining is used to differentiate gram positive (that doesnt have outer membrane) and gram negative (cell wall+outer membrane) bacteria.
If your organisms were all blue with pink spots what is the Gram reaction if your organisms were all pink but had some blue spots what is the Gram reaction?
Gram positive cells take up the crystal violet, which is then fixed in the cell with the iodine mordant. This forms a crystal-violet iodine complex which remains in the cell even after decolorizing. It is thought that this happens because the cell walls of gram positive organisms include a thick layer of protein-sugar complexes called peptidoglycans. This layer makes up 60-90% of the gram positive cell wall. Decolorizing the cell causes this thick cell wall to dehydrate and shrink, which closes the pores in the cell wall and prevents the stain from exiting the cell. At the end of the gram staining procedure, gram positive cells will be stained a purplish-blue color. Gram negative cells also take up crystal violet, and the iodine forms a crystal violet-iodine complex in the cells as it did in the gram positive cells. However, the cell walls of gram negative organisms do not retain this complex when decolorized. Peptidoglycans are present in the cell walls of gram negative organisms, but they only comprise 10-20% of the cell wall. Gram negative cells also have an outer layer which gram positive organisms do not have; this layer is made up of lipids, polysaccharides, and proteins. Exposing gram negative cells to the decolorizer dissolves the lipids in the cell walls, which allows the crystal violet-iodine complex to leach out of the cells. This allows the cells to subsequently be stained with safranin. At the end of the gram staining procedure, gram negative cells will be stained a reddish-pink color. Remember:
Are human cells gram positive?
Human cells are Gram-negative because they do not contain certain structures. More simply, if they don't have a cell wall they cannot be Gram-positive.
Can prokaryotic cells be either gram positive or gram negative?
The chief component of the Prokaryotic cell wall is peptidoglycan. Peptidoglyon readily absorbs stain so all Prokaryotic cells are gram postive.
Why is gram staining not usually applied to eucaryotes?
Gram staining is commonly used on prokaryotic bacteria to determine if their cell wall is gram negative or gram positive. Their cell wall is made of peptidoglycan. It responds well and is stained in certain ways depending on the structure (wether it's gram positive or negative). Animal eukaryotic cells don't even have a cell wall to be stained. In the case of eukaryotic plant cells, that do have cell walls, their cell wall is not made of peptidoglycan. In any case, there is no such thing as gram positive, or gram negative, cell walls in eukaryotes. There would be no point in the test, since it's specifically designed for prokaryotic cells.
Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
A cells boundary is called the what?
In animal cells, the plasma membrane forms the outer boundary of the cell. In fungi and plant cells, a cell wall exists outside the plasma membrane. Gram-positive bacteria have outer cell walls, while Gram-negative bacteria possess inner and outer plasma membranes.
What is the current theory about the mechanism of the gram stain?
The gram stain is a basic differential stain used to determine if a bacterial cell is gram positive or negative. Gram positive cells have a thick peptidoglycan layer that will trap the crystal violet iodine crystalls and apear purple. Gram negative cells only have a thin peptidoglycan layer that allows the crystals to diffuse out of the cell and will only be seen with the application of a counterstain, such as safranin which turns the cells pink.
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
What causes a stain to adhere to bacterial cells?
In a gram positive stain it is because the cell's cell wall is made up of peptioglycan
If iodine step missed in gram stain what will happen?
Iodine (I - or I3 - ) interacts with CV+ and forms large complexes of crystal violet and iodine (CV-I) within the inner and outer layers of the cell. Iodine is often referred to as a mordant, but is a trapping agent that prevents the removal of the CV-I complex and, therefore, color the cell.[10]
What are the three basic Theories on the principle of gram staining reactions?
The gram stain is a basic differential stain used to determine if a bacterial cell is gram positive or negative. Gram positive cells have a thick peptidoglycan layer that will trap the crystal violet iodine crystalls and apear purple. Gram negative cells only have a thin peptidoglycan layer that allows the crystals to diffuse out of the cell and will only be seen with the application of a counterstain, such as safranin which turns the cells pink.
