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It generates larger amounts of dna from tiny amounts
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What is the full form PCR?
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What does the acronym rt pcr stand for?
RT-PCR stands for Reverse-Transcription-Polymerase Chain Reaction. It is used in labratories to generate many copies of a DNA sequence. There are other abbreviations close to this as well.
What is the meaning of optimization of PCR with respect to specific parameters?
optimization means providing the perfect conditions for taq pol to bind and thus a smooth reactionb that amplifies our sample................this includes optimizing the amplification temp, the pH of buffer , the purity chech of the DNA that's to be amplified,adding correct amount of enzyme etc...............for additional information do refer to sambrook and rusell lab manual page 155
Role of magnesium chloride in PCR?
mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr
What companies offer real-time PCR data analysis?
Companies which offer real-time PCR data analysis are mainly computer and technology companies like Intel. You could find other companies by researching on-line.
What is the role of pcr in laser toner?
Pcr serves to transfer an electric charge to the surface of photo conductor drum located in toner cartrige, the pcr is in contact with opc drum as drum turns,any loosr deposits on pcr transfarred to the drum
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
Why you go for bulk pcr?
when we want to obtain huge amount of DNA ,for further analysis like restriction digestion,ligation or transformtaion we perform bulk pcr where we make reaction mixture like 100 ul or so
What is a PCR test used to identify?
A PCR test amplifies a single or few copies of DNA and creates potentially thousands or millions of copies. The most common reasons are for cloning, diagnosis of hereditary disease, genetic fingerprints, and analysis of genes.
How could pcr be used to diagnose diseases or other medical conditions?
PCR itself cannot be used to diagnose a disease; it is only useful for amplifying a DNA sample. Molecular analysis can only be done with subsequent techniques, such as electrophoresis and DNA sequencing.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
What is the role of dNTP in PCR?
dNTP's are the building blocks for new strands.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What does the PCR do?
Polymerase chain reaction (PCR) is an efficient and cost-effective molecular tool to copy, detect and amplify small segments of DNA or RNA. With decades of development since it’s firstly discovered by the scientist Kary Mullis, several modifications of PCR methods have been developed to enhance the utility of it in diagnostic settings based on their applications.
