By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
Placing the mixture above the solvent level in chromatography allows the solvent to travel up the stationary phase through capillary action, carrying the components of the mixture at different rates based on their interactions with the stationary phase. This separation process helps to distinguish and isolate the different components of the mixture.
above
The solubility of a gas in a solvent is directly proportional to the partial pressure of that gas above the solvent.
Submerging the chromatography paper above the pencil line would cause the solvent to rise above the line, potentially leading to the ink or pigment above the line to dissolve and be carried upwards with the solvent. This can result in the separation of different components within the ink or pigment.
Drawing a pencil line above the level of the solvent ensures that the compound being separated does not dissolve directly in the solvent, preventing interference with the separation process. This helps maintain a distinct separation between the solvent front and the compounds being separated, leading to more accurate results during chromatography.
It's placed above the injury site
A tomb is a structure that typically is above ground in which the casket is placed. A grave is a hole in the ground in which the casket is placed. A tomb stone is a structure placed above a grave to mark the location of the grave.
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If the solvent is above the level of the initial sample spots in a chromatography experiment, it can lead to the sample being dissolved or dispersed throughout the solvent, causing smudging of the spots and inaccurate separation of components. This can result in poor resolution and difficulty in analyzing the components present in the sample.
Best placed 50 - 100mm above the drain of the steriliser.
It is placed 2 to 4 inches above the wound.