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The membrane binds proteins unspecifically (it will bind any).
Western blotting uses antibodies to probe the proteins bound to the membrane for the one you are testing for. The antibodies bind specifically to a particular protein that you have chosen and contain a label to allow identification of them on the membrane. In this way you can see whether or not the membrane, and therefore the tissue you are testing, contains a particular protein.
Antibodies are proteins. This means the antibody would bind anywhere on the membrane, as well as on the protein you're looking for, rendering the results worthless (it will ALWAYS show positive). To stop the antibodies binding to the membrane, so that they only bind to the protein (if it exists) the membrane must be blocked (completely filled with protein) so that there is nowhere on the membrane for the antibody to bind.
Since milk contains a generic mix of a relatively high concentration of known proteins, it is used to block the parts of the membrane that don't already have protein on so that the antibodies won't bind there. As it is very cheap and readily available, it is a good source of protein to use. It is unlikely that a Western blot would be needed for any protein that is contained in milk, but if that is the case an alternative mixture can be used.
What else can I help you with?
Who provides the milk for Western Family Food?
meadow gold provides the milk for western family
Milk known as in western countries?
Probably milk.
What is collective noun for milk?
block
What is a milk block that is used to stop chickens from pecking each other?
A milk block is a nutritional supplement made of dried milk protein that is used to prevent chickens from pecking each other. Chickens may peck at each other due to stress, boredom, or overcrowding, and offering a milk block can provide distraction and enrichment while also supplying additional protein to their diet.
Is milk gravy northern or southern?
western
What is the Collective noun for milk coupons?
block
Can you freeze chocolate milk in paper milk container?
No, you cannot freeze chocolate milk to make chocolate. The chocolate syrup and the milk are just being blended together and frozen into a block of chocolate milk. You can't derive the chocolate from the milk, either, so it is impossible.
Can a gummy bear absorb milk?
Yes, a gummy bear can absorb milk due to osmosis. When placed in a solution like milk, the gummy bear will absorb the liquid through its membrane, causing it to swell in size as it takes in the milk.
Why is milk sold in containers that block out light?
Milk is sold in containers that block out light to prevent oxidation and degradation of its nutrients, particularly vitamin A and riboflavin, which can break down when exposed to light. Light exposure can also lead to off-flavors and spoilage in milk.
Which Western Hemisphere country leads the world in milk and butter production?
chad
How are western and eastern dragons the same?
Diet: both enjoy milk ( for the western dragon it makes it drunk and sleepy so people can kill it).
Clock that is a shape of the milk chocolate block with Cadbury wrote on it in gold?
Yes there is such a thing I have one.
