No, in the anime Yusei Fudo never becomes a Dark Signer.
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the 5th signer used to be rex goodwin (a guy with the mark of a signer and a mark of a dark signer on each arms) but after the other signers defeated the dark signers and yusei, jack and crow defeated rex goodwin crow (who was actually not a signer) became a signer after rex was defeated in a duel by getting yuseis old mark and yusei gettin rex goodwins mark so in another way: rex used to be a signer but he was defeated so crow became the 5th signer try and watch the series a bit more
Roman Goodwin is origeonally the 5th signer but when he and his brother rex go into the afterlife, Romans head sign goes onto yusei and yuseis sign goes onto crow in the final battle with rex goodwin
Carly Carmine became a Dark Signer in episode 39.
Kalin kessler was yusei, jack, and crow's friend in yu gi oh 5d's who was also the leader of their duel gang who destroyed every crimnal duel gang in the satellite. He then went too far and tried to take down sector security but he was stopped with the help of yusei, jack, and crow. He thought that they were traitors and took a grudge on yusei. While in the facility he became a dark signer and escaped to end his vendetta with yusei. After the dark signer crisis ended he became their friend again.
OK the fifth signer is Goodwin's brother but he chose to be a dark signer so he cut his hands of and gave it to his brother Rex Goodwin and the Goodwin became a dark signer and so he took out i has mechanical arm and put in the his brother's arm so he could be a man posses two signs during the battle with Rex Goodwin and yusei, jack, crow the crimson dragon chose new signer the dragon gave yusei the most important part of the signs the head of the crimson dragon and so yusei's old sign was given to crow now crow is the fifth signer if you don't believe me watch episode 64 of yu-gi-oh 5ds witch is in japense