The Ames test is a biological assay used to assess the mutagenic potential of chemical compounds. It involves exposing strains of bacteria, usually Salmonella typhimurium, to the test substance and assessing the rate of mutations that enable the bacteria to grow in the absence of histidine. By comparing the number of mutant colonies formed with a control, researchers can determine whether the substance is a mutagen. The test is a valuable tool in toxicology and cancer research for identifying potential carcinogens.
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Scott Ames is a prominent figure in the field of academia, particularly known for his work in the area of psychology and behavior analysis. He has contributed to research on topics such as motivation, learning, and behavioral interventions. Additionally, Ames has published numerous articles and has been involved in educational initiatives aimed at improving teaching and learning practices. His work emphasizes the importance of understanding psychological principles to enhance educational outcomes.
Harry Ames was born in 1896.
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The Ames test identifies chemical mutagens.
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The substances that are test positive with Ames test may or may not be carcinogenic for humans. Similarly, some substances that cause cancer in laboratory animals do not give a positive result Ames test. It is not possible to decide the carcinogenicity of any substances only depending on the Ames test.
Ames test would typically be carried out in the field of biology, more specifically toxicology. The Ames test involves testing genetics, which involves taking a sample and examining it closely in a laboratory.
The test used for detecting chemicals with carcinogenic potential is the Ames test. This test uses bacteria to detect mutations induced by the chemical being tested, which can indicate its carcinogenic potential.
Ames test is used to test whether a certain substance is mutagenic. Liver extract is optionally added to simulate the effect of metabolism, as some compounds, like benzo[a]pyrene, are not mutagenic themselves but their metabolic products are.
The Ames test detects mutagens by measuring the rate of mutations in specific strains of bacteria, typically Salmonella typhimurium, which are unable to synthesize histidine due to a mutation. When exposed to potential mutagens, any revertant colonies that regain the ability to synthesize histidine indicate that mutations have occurred. The test compares the number of revertant colonies on plates with and without the test substance to determine if the substance is mutagenic. A significant increase in revertants suggests that the substance may be a mutagen.
The control component in an Ames Test typically includes a negative control (no chemical exposure) to ensure background levels of spontaneous mutations, and a positive control (known mutagen) to validate the sensitivity of the assay. These controls help researchers interpret the results of the test by providing a baseline for comparison.
Blanche Ames Ames died in 1969.
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The address of the Ames Public Library is: 515 Douglas Ave, Ames, 50010 6215
Pauline Ames Plimpton has written: 'The ancestry of Blanche Butler Ames and Adelbert Ames'