1.
DMSO
2.
Ethylene glycol
3.
Glycerol
4.
2-Methyl-2,4-pentanediol
5.
Propylene glycol
6.
Sucrose
7.
Methylcellosolve
8.
Dimethylformamide
Rock-Type moves are weak against Fighting-Types, Ground-Types, and Steel-Types. Rock-Type Pokémon are weak against Water-Types, Grass-Types, Fighting-Types, Ground-Types, and Steel-Types.
No. Fire-Types are strong against Grass-Types, Bug-Types, Ice-Types, and Steel-Types. Dark-Types are weak against Bug-Types and Fighting-Types.
When attacking, Ghost-Type Pokémon are strong against Psychic-Types and other Ghost-Types, weak against Dark-Types and Steel-Types, and useless against Normal-Types. When being attacked, Ghost-Type Pokémon are strong against Bug-Types and Poison-Types, weak against Dark-Types and other Ghost-Types, and invincible against Normal-Types and Fighting-Types (barring the use of a move like Foresight).
Pupitar is a Rock- and Ground-Type, so it is weak against Steel-Types, Ground-Types, Fighting-Types, and Ice-Types, and exceptionally weak against Water-Types and Grass-Types.
types of audit approach
B
One uses a chemical (or mixture of chemicals) known as a cryoprotectant. There are a number of different cryoprotectants. The major obstacle is that many of them are toxic to human biology. Using a mixture generally reduces the toxicity since different cryoprotectants are toxic in different ways. One tries to come up with a mixture that doesn't have a toxic amount of any single cryoprotectant. Some cryprotectants include glycerol, gycols (propylene and ethylene glycols), Dimethyl sulfoxide (DMSO, a common solvent in organic chemistry) and others.
DMSO (dimethyl sulfoxide) is commonly used as a cryoprotectant for freezing cells, while glycerol is often used as a stabilizer for enzymes and proteins. The choice between DMSO and glycerol depends on the specific application and the stability requirements of the biological material being used.
Equilibration is an important step in cryopreservation as it allows the cells or tissues to adjust to the cryoprotectant solution before freezing. This process helps to minimize osmotic stress and damage to the cells during freezing and thawing. Proper equilibration can improve cell survival rates and maintain cell functionality after cryopreservation.
Recombinant organisms can be preserved by storing them in a cryoprotectant solution and freezing them at ultra-low temperatures using cryopreservation techniques. Another method is to preserve them in a lyophilized (freeze-dried) state, which involves removing water from the organism and storing it at a low temperature. Regular sub-culturing and maintenance of the organism's growth conditions are also important for long-term preservation.
Glycerol can act as a cryoprotectant, stabilizing cell membranes during freezing and thawing processes. It can also disrupt hydrogen bonding within the lipid bilayer, potentially causing leakage of cellular contents and altering membrane structure and function. Additionally, glycerol can help maintain membrane fluidity by interacting with phospholipid molecules.
fire types and grass types and flying types can and bug types can and dragon types can and poison types can and steel types can
Integer types, floating point types, Boolean types, character types, string types, pointer and array types, reference types, enumeration and other user-defined types.
Rock-Type moves are weak against Fighting-Types, Ground-Types, and Steel-Types. Rock-Type Pokémon are weak against Water-Types, Grass-Types, Fighting-Types, Ground-Types, and Steel-Types.
Bug-Types are strong against Psychic-Types, Dark-Types, and Grass-Types.
First trainer has ice types and water types, Second trainer has rock types and fighting types, Third trainer has ghost types and poison types, Fourth trainer has dragon types, Last trainer has all around types meaning there all different types.
No. Fire-Types are strong against Grass-Types, Bug-Types, Ice-Types, and Steel-Types. Dark-Types are weak against Bug-Types and Fighting-Types.