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Microbes protect themselves from their antibiotics by producing their own resistance factors. As discussed in later chapters, microbes may synthesize pumps to pump the antibiotics out; or they may make altered versions of the target macromolecule, such as the ribosome subunit; or they may make enzymes to cleave the antimicrobial substance.

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rebecca lowe

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Q: How do you build a hay infusion ecosystem?
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How do you prepare a hay infusion?

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Does hay infusion use true motility or brownian movement?

Hay infusion consists of microorganisms like protozoa. Most of the protozoa are motile in nature since they have locomotive structures like flagella, cilia and pseudopods that's why the organisms that can be found in hay infusion generally moves.


What are the protozoans that you have observed from the hay infusion?

oxygen breathers


What is the purpose of hay infusion?

A hay infusion is a culture made from water collected from a pond, lake, stream or puddle.


Why is that the microorganisms in the hay infusion are not stained?

Microorganisms in the hay infusion are not stained because the microorganisms are gram negative and if we are trying to use stain them, only gram positive organisms can pick up.


Why is the dark hay infusion have the most bacteria?

Bacteria grow better in the dark.


What is the connection between Leeuwenhoek and the hay infusion experiment?

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Will the ecosystem recover after the oil spill?

It could take the ecosystem years and possibly decades to recover from such an infusion of oil and gas.


From where did the organisms in the infusion of hay and peppercorn come from?

They are present as cysts on the surface of the infusion material. Wetting the cysts liberates the organisms, which grow, divide and re-encyst as a part of their life cycles. Many of them are present in the air we breathe as cysts as well.


Is hay infusion a prokaryotic or a eukaryotic?

Hay infusion is a method of making a suitable home for your microorganisms to grow, this is achieved by boiling hay with water. This results to a broth, rich with carbohydrates and other "food" for your microorganisms extracted from the hay you used. Now, to make your microorganisms grow in numbers in that infusion, you need to get a little amount of the sample that you want to test for the presence of microorganisms. Example, getting a few milliliters of pond water, then pouring it to your hay-infusion. We will leave this for 24-48 hours so that the microorganisms will be able to multiply and grow in your infusion. Now, regarding the question on prokaryotic and eukaryotic cells... These two are actually the types of cells animals may have. Prokaryotic cells are said to be primitive and are commonly seen in bacteria, while eukaryotic cells are more advanced cells seen in higher forms of life like us. However, in the infusion you prepared, after pouring the sample that you want to be tested, we will see a variety of prokaryotic and eukaryotic microorganisms swimming about. Both types are present and it's a matter of identifying one from the other.


What type of organisms will be more prevalent in the hay infusion incubated in the light than in the hay infusion incubated in the dark?

The type of organisms that would be prevalent in a lighted incubator would be plants or other organisms that need light to survive. Some types of bacteria would grow well in the unlighted incubator.


Why do you have to wait for a week or two before examining the hay infusion?

Because at the start there are no aquatic organisms in the water. Spores from the air land in the water and find that it suits them very well. They emerge from the spores. At this stage you would not see them. Now they feed on the bacteria that are breaking down the hay. They multiply while there is no limit on the food available to them. Now if you examine the hay infusion you will find them in large numbers.