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Microbes protect themselves from their antibiotics by producing their own resistance factors. As discussed in later chapters, microbes may synthesize pumps to pump the antibiotics out; or they may make altered versions of the target macromolecule, such as the ribosome subunit; or they may make enzymes to cleave the antimicrobial substance.

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rebecca lowe

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Q: How do you build a hay infusion ecosystem?
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What are the protozoans that you have observed from the hay infusion?

I have observed Paramecium, Amoeba, Euglena, and Stentor protozoans in the hay infusion. These organisms are commonly found in freshwater habitats and are able to thrive in the nutrient-rich environment provided by the hay infusion.


Why is that the microorganisms in the hay infusion are not stained?

Microorganisms in the hay infusion may not stain because they have a complex cell wall structure that prevents the stain from penetrating. Additionally, the staining process may not be effective on certain types of microorganisms present in the hay infusion.


Does hay infusion use true motility or brownian movement?

Hay infusion uses both true motility and Brownian movement. True motility is the active movement of microorganisms, such as bacteria and protists, whereas Brownian movement refers to the random movement of particles due to collisions with surrounding molecules. In a hay infusion, you can observe both types of movements among the microorganisms present in the sample.


How do you prepare a hay infusion?

To prepare a hay infusion, place a handful of hay in a container of water and let it sit in a warm, sunny spot for a few days to allow the hay to decompose. This creates an environment for microorganisms to grow. After a few days, strain out the hay and use the liquid as a culture medium for observing microorganisms under a microscope.


What is the comparison of sizes and shapes of microorganisms observed in the light and dark hay infusion?

Microorganisms observed in the light hay infusion tend to be larger and more diverse in shape compared to those in the dark hay infusion. This is because light stimulates the growth of photosynthetic microorganisms that are generally larger in size and have different shapes than those found in the dark.


Will the ecosystem recover after the oil spill?

It could take the ecosystem years and possibly decades to recover from such an infusion of oil and gas.


Why do microorganism thrive in hay infusion?

Microorganisms thrive in hay infusions because the hay provides a source of nutrients and organic matter for them to feed on. The water in the infusion creates a suitable environment for growth, with optimal temperature and pH levels. Additionally, the organic material in hay can promote microbial diversity and abundance in the infusion.


What is the purpose of hay infusion?

Hay infusion is used as a rich nutrient source for culturing microorganisms in a laboratory setting. It provides a diverse variety of organic material for organisms to feed on, promoting the growth of a wide range of microbes. This can help in studying microbial diversity and ecology.


Is hay infusion a prokaryotic or a eukaryotic?

Hay infusion is a method of making a suitable home for your microorganisms to grow, this is achieved by boiling hay with water. This results to a broth, rich with carbohydrates and other "food" for your microorganisms extracted from the hay you used. Now, to make your microorganisms grow in numbers in that infusion, you need to get a little amount of the sample that you want to test for the presence of microorganisms. Example, getting a few milliliters of pond water, then pouring it to your hay-infusion. We will leave this for 24-48 hours so that the microorganisms will be able to multiply and grow in your infusion. Now, regarding the question on prokaryotic and eukaryotic cells... These two are actually the types of cells animals may have. Prokaryotic cells are said to be primitive and are commonly seen in bacteria, while eukaryotic cells are more advanced cells seen in higher forms of life like us. However, in the infusion you prepared, after pouring the sample that you want to be tested, we will see a variety of prokaryotic and eukaryotic microorganisms swimming about. Both types are present and it's a matter of identifying one from the other.


Why is the dark hay infusion have the most bacteria?

The dark hay infusion may have the most bacteria because the lack of light allows for more bacterial growth, and the decomposition of organic matter in the hay provides nutrients for bacteria to thrive. Additionally, the darkness creates a more stable environment for bacterial populations to flourish.


From where did the organisms in the infusion of hay and peppercorn come from?

They are present as cysts on the surface of the infusion material. Wetting the cysts liberates the organisms, which grow, divide and re-encyst as a part of their life cycles. Many of them are present in the air we breathe as cysts as well.


What is the connection between Leeuwenhoek and the hay infusion experiment?

Antonie van Leeuwenhoek is credited with improving the microscope, enabling him to observe microorganisms in water samples, including from a hay infusion. His discoveries were crucial in the development of microbiology and helped establish the existence of microorganisms in the natural environment.